School of Life Science and Technology, Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandong Universities Key Laboratory of Biopharmaceuticals, Weifang Medical University, Weifang, Shandong, 261053, People's Republic of China.
School of Basic Medicine, Weifang Medical University, Weifang, Shandong, 261053, People's Republic of China.
Int J Nanomedicine. 2021 Jul 13;16:4769-4780. doi: 10.2147/IJN.S304702. eCollection 2021.
The treatment of ()-infected wounds is difficult. It causes extreme pain to tens of thousands of patients and increases the cost of medical care. The antimicrobial peptide OH-CATH30 (OH30) has a good killing activity against and can play a role in accelerating wound healing and immune regulation. Therefore, it shows great potential for wound healing.
The aim of this study was to overcome the short half-life and easy enzymolysis of OH30 by using graphene oxide conjugated with polyethylene glycol to load OH30 (denoted as PGO-OH30), as well as to evaluate its effect on wounds infected by
PGO-OH30 nanoparticles were prepared by π-π conjugation and characterized. Their cell cytotoxicity, cell migration, infectious full-thickness dermotomy models, and histopathology were evaluated.
Characterization and cytotoxicity experiments revealed that the PGO-OH30 drug-delivery system had good biocompatibility and excellent drug-delivery ability. Cell-migration experiments showed that PGO-OH30 could promote the migration of human immortalized keratinocytes (HaCaT) cells compared with the control group (<0.05). In a mouse model of skin wound infection, PGO-OH30 accelerated skin-wound healing and reduced the amount of in wounds compared with the control group (<0.05). In particular, on day 7, the number of was 100 times lower in the PGO-OH30 group than in the control group.
The PGO-OH30 drug-delivery system had good biocompatibility and excellent drug-delivery ability, indicating its good therapeutic effect on a skin wound-infection model.
（）感染伤口的治疗较为困难，给成千上万的患者带来极大的痛苦，并增加医疗成本。抗菌肽 OH-CATH30（OH30）对有很好的杀伤活性，并且能够在加速伤口愈合和免疫调节方面发挥作用。因此，它在伤口愈合方面显示出巨大的潜力。
本研究旨在通过使用氧化石墨烯与聚乙二醇偶联来负载 OH30（命名为 PGO-OH30）来克服 OH30 的半衰期短和易酶解的问题，并评估其对（）感染伤口的疗效。
通过 π-π 键合制备 PGO-OH30 纳米颗粒，并对其进行表征。评估其细胞毒性、细胞迁移、感染性全层皮肤切开模型和组织病理学。
表征和细胞毒性实验表明，PGO-OH30 递药系统具有良好的生物相容性和优异的递药能力。细胞迁移实验表明，与对照组相比，PGO-OH30 能够促进人永生化角质形成细胞（HaCaT）的迁移（<0.05）。在皮肤伤口感染的小鼠模型中，与对照组相比，PGO-OH30 加速了皮肤伤口愈合，并减少了伤口中的（）数量（<0.05）。特别是在第 7 天，PGO-OH30 组中的数量比对照组低 100 倍。
PGO-OH30 递药系统具有良好的生物相容性和优异的递药能力，表明其对皮肤伤口感染模型具有良好的治疗效果。
