• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

探索香茅醇的肝脏保护特性：以及对乙醇诱导的HepG2细胞损伤的研究。

Exploring the hepatoprotective properties of citronellol: and studies on ethanol-induced damage in HepG2 cells.

作者信息

Malik Muhammad Nasir Hayat, Abid Iqra, Ismail Sana, Anjum Irfan, Qadir Halima, Maqbool Tahir, Najam Komal, Ibenmoussa Samir, Bourhia Mohammed, Salamatullah Ahmad Mohammad, Wondmie Gezahign Fentahun

机构信息

Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan.

Department of Basic Medical Sciences, Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan.

出版信息

Open Life Sci. 2024 Sep 9;19(1):20220950. doi: 10.1515/biol-2022-0950. eCollection 2024.

DOI:10.1515/biol-2022-0950
PMID:39290493
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11406226/
Abstract

Citronellol (CT) is a monoterpene alcohol present in the essential oil of plants of the genus and exhibits diverse pharmacological activities. The aim of the current study was to investigate the hepatoprotective potential of CT against ethanol-induced toxicity in HepG2 cell lines. Silymarin (SIL) was used as a standard drug. MTT, crystal violet assay, DAPI, and PI staining were carried out to assess the effect of ethanol and CT on cell viability. RT-PCR determined the molecular mechanisms of hepatoprotective action of CT. CT ameliorated cell viability and restricted ethanol-induced cell death. DAPI and PI staining showed distinct differences in cell number and morphology. Less cell viability was observed in the diseased group obviously from strong PI staining when compared to the CT- and SIL-treated group. Moreover, CT showed downregulation of interleukin (IL-6), transforming growth factor-beta 1 (TGF-β1), collagen type 1 A 1 (COL1A1), matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) and glutathione peroxidase-7 (GPX-7) levels. Molecular docking studies supported the biochemical findings. It is concluded that the cytoprotective activity of CT against ethanol-induced toxicity might be explained by its anti-inflammatory, immunomodulatory, and collagen-regulating effects.

摘要

香茅醇（CT）是一种存在于某些植物属精油中的单萜醇，具有多种药理活性。本研究的目的是探讨CT对乙醇诱导的HepG2细胞系毒性的肝脏保护潜力。水飞蓟素（SIL）用作标准药物。采用MTT法、结晶紫测定法、DAPI和PI染色来评估乙醇和CT对细胞活力的影响。逆转录聚合酶链反应（RT-PCR）确定了CT肝脏保护作用的分子机制。CT改善了细胞活力并限制了乙醇诱导的细胞死亡。DAPI和PI染色显示细胞数量和形态存在明显差异。与CT和SIL处理组相比，病变组中明显可见较强的PI染色，细胞活力较低。此外，CT显示白细胞介素（IL-6）、转化生长因子-β1（TGF-β1）、I型胶原蛋白A1（COL1A1）、基质金属蛋白酶-1（MMP-1）、金属蛋白酶组织抑制剂-1（TIMP-1）和谷胱甘肽过氧化物酶-7（GPX-7）水平下调。分子对接研究支持了这些生化结果。得出的结论是，CT对乙醇诱导毒性的细胞保护活性可能归因于其抗炎、免疫调节和胶原蛋白调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/7caecdb532aa/j_biol-2022-0950-fig009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/f1504c0928b6/j_biol-2022-0950-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/9c4ee2547eb7/j_biol-2022-0950-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/41b1aa353ef5/j_biol-2022-0950-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/037e50d4a3d5/j_biol-2022-0950-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/a39b8286f8b2/j_biol-2022-0950-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/5b0c63d81f26/j_biol-2022-0950-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/9c72f9f166fa/j_biol-2022-0950-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/b5d16dca6121/j_biol-2022-0950-fig008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/7caecdb532aa/j_biol-2022-0950-fig009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/f1504c0928b6/j_biol-2022-0950-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/9c4ee2547eb7/j_biol-2022-0950-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/41b1aa353ef5/j_biol-2022-0950-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/037e50d4a3d5/j_biol-2022-0950-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/a39b8286f8b2/j_biol-2022-0950-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/5b0c63d81f26/j_biol-2022-0950-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/9c72f9f166fa/j_biol-2022-0950-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/b5d16dca6121/j_biol-2022-0950-fig008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb85/11406226/7caecdb532aa/j_biol-2022-0950-fig009.jpg

相似文献

1
Exploring the hepatoprotective properties of citronellol: and studies on ethanol-induced damage in HepG2 cells.探索香茅醇的肝脏保护特性：以及对乙醇诱导的HepG2细胞损伤的研究。
Open Life Sci. 2024 Sep 9;19(1):20220950. doi: 10.1515/biol-2022-0950. eCollection 2024.
2
Hepatoprotective potency of Litsea glutinosa (L.) C.B. Rob. leaf methanol extract on HO-induced toxicity in HepG2 cells.潺槁木姜子叶甲醇提取物对HepG2细胞中HO诱导毒性的肝保护作用。
J Ethnopharmacol. 2023 Mar 25;304:116076. doi: 10.1016/j.jep.2022.116076. Epub 2022 Dec 22.
3
Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats.没药属植物提取物对四氯化碳诱导的 HepG2 细胞和大鼠肝毒性的抗氧化和保肝作用。
J Ethnopharmacol. 2013 Dec 12;150(3):973-81. doi: 10.1016/j.jep.2013.09.048. Epub 2013 Oct 17.
4
Artabotrys odoratissimus Bark Extract Restores Ethanol Induced Redox Imbalance and Toxicity in Hepatocytes and In Vivo Model.鸡屎藤叶提取物恢复乙醇诱导的肝细胞氧化还原失衡和毒性及在体内模型中的作用。
Appl Biochem Biotechnol. 2023 May;195(5):3366-3383. doi: 10.1007/s12010-022-04275-y. Epub 2022 Dec 31.
5
Rat pancreatic stellate cells secrete matrix metalloproteinases: implications for extracellular matrix turnover.大鼠胰腺星状细胞分泌基质金属蛋白酶：对细胞外基质周转的影响。
Gut. 2003 Feb;52(2):275-82. doi: 10.1136/gut.52.2.275.
6
Downregulation of matrix metalloproteinase-9 by melatonin during prevention of alcohol-induced liver injury in mice.褪黑素在预防小鼠酒精性肝损伤中对基质金属蛋白酶-9 的下调作用。
Biochimie. 2011 May;93(5):854-66. doi: 10.1016/j.biochi.2011.02.007. Epub 2011 Feb 24.
7
Heliconia rostrata rhizomes mitigate chemical-induced liver injury by debilitating oxidative stress in HepG2 cells and rats.红鹤蕉根茎通过削弱 HepG2 细胞和大鼠的氧化应激来减轻化学性肝损伤。
J Ethnopharmacol. 2021 Jul 15;275:114113. doi: 10.1016/j.jep.2021.114113. Epub 2021 Apr 20.
8
Anti-inflammatory/anti-fibrotic effects of the hepatoprotective silymarin and the schistosomicide praziquantel against Schistosoma mansoni-induced liver fibrosis.水飞蓟素（一种保肝药）和驱虫药吡喹酮抗曼氏血吸虫诱导的肝纤维化的抗炎/抗纤维化作用。
Parasit Vectors. 2012 Jan 11;5:9. doi: 10.1186/1756-3305-5-9.
9
Investigating the Antifibrotic Effects of β-Citronellol on a TGF-β1-Stimulated LX-2 Hepatic Stellate Cell Model.研究β-香茅醇对 TGF-β1 刺激的 LX-2 肝星状细胞模型的抗纤维化作用。
Biomolecules. 2024 Jul 5;14(7):800. doi: 10.3390/biom14070800.
10
Effects of silymarin and pentoxifylline on matrix metalloproteinase-1 and -2 expression and apoptosis in experimental hepatic fibrosis.水飞蓟素和己酮可可碱对实验性肝纤维化中基质金属蛋白酶-1和-2表达及细胞凋亡的影响
Curr Ther Res Clin Exp. 2008 Dec;69(6):488-502. doi: 10.1016/j.curtheres.2008.12.003.

引用本文的文献

1
Computational analysis of polymorphic residues in maltose and maltotriose transporters of a wild strain.野生菌株麦芽糖和麦芽三糖转运蛋白中多态性残基的计算分析
Open Life Sci. 2025 Apr 16;20(1):20251080. doi: 10.1515/biol-2025-1080. eCollection 2025.

本文引用的文献

1
Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane.柚皮苷、二十烷和二十八烷的抗氧化、伤口愈合潜力及计算机评估。
Molecules. 2023 Jan 20;28(3):1043. doi: 10.3390/molecules28031043.
2
Hepatoprotective Potential of Malaysian Medicinal Plants: A Review on Phytochemicals, Oxidative Stress, and Antioxidant Mechanisms.马来西亚药用植物的保肝潜力：植物化学物质、氧化应激和抗氧化机制的综述。
Molecules. 2022 Feb 24;27(5):1533. doi: 10.3390/molecules27051533.
3
Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Root Extract in Rats.
乙醇诱导的肝毒性以及富含γ-氨基丁酸的发酵根提取物对大鼠酒精代谢的调节作用
Foods. 2021 Oct 8;10(10):2381. doi: 10.3390/foods10102381.
4
Molecular docking analysis of stachydrine and sakuranetin with IL-6 and TNF-α in the context of inflammation.水苏碱和樱花素与白细胞介素-6和肿瘤坏死因子-α在炎症背景下的分子对接分析
Bioinformation. 2021 Feb 28;17(2):363-368. doi: 10.6026/97320630017363. eCollection 2021.
5
Different roles of interleukin 6 and interleukin 11 in the liver: implications for therapy.白细胞介素6和白细胞介素11在肝脏中的不同作用：对治疗的启示
Hum Vaccin Immunother. 2020 Oct 2;16(10):2357-2362. doi: 10.1080/21645515.2020.1761203. Epub 2020 Jun 12.
6
GPx7 ameliorates non-alcoholic steatohepatitis by regulating oxidative stress.GPx7 通过调节氧化应激改善非酒精性脂肪性肝炎。
BMB Rep. 2020 Jun;53(6):317-322. doi: 10.5483/BMBRep.2020.53.6.280.
7
TGF-β1 accelerates the hepatitis B virus X-induced malignant transformation of hepatic progenitor cells by upregulating miR-199a-3p.TGF-β1 通过上调 miR-199a-3p 加速乙型肝炎病毒 X 诱导的肝前体细胞恶性转化。
Oncogene. 2020 Feb;39(8):1807-1820. doi: 10.1038/s41388-019-1107-9. Epub 2019 Nov 18.
8
A comprehensive review on hepatoprotective and nephroprotective activities of chrysin against various drugs and toxic agents.金雀异黄素对各种药物和毒物的肝保护和肾保护作用的综合评价。
Chem Biol Interact. 2019 Aug 1;308:51-60. doi: 10.1016/j.cbi.2019.05.010. Epub 2019 May 11.
9
Citronellol, a monoterpene alcohol with promising pharmacological activities - A systematic review.香茅醇，一种具有广阔药理活性的单萜醇——系统评价。
Food Chem Toxicol. 2019 Jan;123:459-469. doi: 10.1016/j.fct.2018.11.030. Epub 2018 Nov 16.
10
Hepatoprotective Effect of Steroidal Glycosides From on Hydrogen Peroxide-Induced Hepatotoxicity in HepG2 Cells.来自[具体来源未给出]的甾体糖苷对过氧化氢诱导的HepG2细胞肝毒性的肝保护作用。
Front Pharmacol. 2018 Jul 23;9:797. doi: 10.3389/fphar.2018.00797. eCollection 2018.