Department of Prosthodontics, School of Dentistry and Dental Research Institute, Seoul National University, 101 Daehak-ro, Jongro-gu, Seoul, 03080, Korea.
Dental Research Institute, Seoul National University, Seoul, Korea.
Int J Implant Dent. 2022 Jun 6;8(1):25. doi: 10.1186/s40729-022-00427-1.
Osseointegration consists of bidirectional bone formation around modified implant surfaces by contact osteogenesis and distance osteogenesis. This study tested whether contact osteogenesis on the surface of a modified titanium (Ti) implant is stimulated by cytokines in the blood.
In the first two types of experiments, sandblasted, large-grit, acid-etched Ti implants and turned Ti tubes were inserted into rabbit tibiae. To exclude the influence of distance osteogenesis, the tubes were inserted into the tibiae, and implants were placed inside the tubes. In a third type of experiment, the implants and tubes were inserted into the rabbit tibiae, and platelet-rich plasma (PRP) or recombinant human bone morphogenetic protein-2 (rhBMP-2) was applied topically. Four weeks after implantation, undecalcified specimens were prepared for histomorphometry. Bone-to-implant contact (BIC) and bone area per tissue (BA) were measured, and the data were analysed using one-way ANOVA at a significance level of 0.05.
When the response of bone to Ti tubes with implants was compared to that without implants (first experiment), little bone formation was found inside the tubes. The mean BIC of implant specimens inside the tubes was 21.41 ± 13.81% in a second experiment that evaluated bone responses to implants with or without Ti tubes. This mean BIC value was significantly lower than that in the implant-only group (without tubes) (47.32 ± 12.09%, P = 0.030). The third experiment showed that rhBMP-2 significantly increased contact osteogenesis on the implant surface, whereas PRP had no effect (mean BIC: 66.53 ± 14.06% vs. 16.34 ± 15.98%, P = 0.004).
Platelet-rich plasma alone is unable to trigger contact osteogenesis on the modified titanium implant surface.
骨整合包括通过接触成骨和距离成骨在改性种植体表面双向形成骨。本研究旨在测试血液中的细胞因子是否会刺激改性钛（Ti）种植体表面的接触成骨。
在前两种实验中，喷砂大颗粒酸蚀 Ti 种植体和车削 Ti 管插入兔胫骨。为了排除距离成骨的影响，将管子插入胫骨，将植入物放置在管子内部。在第三种实验中，将植入物和管子插入兔胫骨，并局部应用富含血小板的血浆（PRP）或重组人骨形态发生蛋白-2（rhBMP-2）。植入 4 周后，制备未脱钙标本进行组织形态计量学分析。测量骨与种植体接触（BIC）和组织骨面积（BA），并使用单向方差分析在 0.05 的显著性水平下对数据进行分析。
当比较带植入物的 Ti 管与不带植入物的骨对 Ti 管的反应（第一项实验）时，发现管内骨形成较少。第二项实验评估了带或不带 Ti 管的植入物的骨反应，结果显示管内植入物标本的平均 BIC 为 21.41±13.81%。该平均值明显低于仅植入物组（无管）（47.32±12.09%，P=0.030）。第三项实验表明，rhBMP-2 显著增加了种植体表面的接触成骨，而 PRP 则没有影响（平均 BIC：66.53±14.06% vs. 16.34±15.98%，P=0.004）。
单独使用富含血小板的血浆无法触发改性钛种植体表面的接触成骨。
