Dubuisson L, Sztark F, Bedin C, Bioulac-Sage P, Balabaud C
Virchows Arch A Pathol Anat Histopathol. 1985;407(3):347-57. doi: 10.1007/BF00710659.
Shunting of portal blood in the rat leads to liver atrophy and to an increase in arterial blood flow with microcirculatory disturbances. The aim of this study was to investigate the effects of these disturbances on the liver sinusoidal barrier (endothelial and perisinusoidal cells) using morphometric techniques. Rats with portacaval anastomosis (PCA) and sham operated pair-fed controls were studied 3 months after the shunt. Sinusoidal volume density in PCA increased but not significantly and the volume density (Vv) of total endothelial (EC) and perisinusoidal cells (PSC) increased by 104.54% compared to sham operated pair-fed rats. The increase of EC Vv was not associated with an increase in surface density (Sv) suggesting a fall in the number of small fenestrations and an increase in cell thickness. This interpretation supports the morphological observations. The increase of PSC Vv was mainly related to the increase in their subendothelial processes Vv and not to that of the cell body Vv. Lipids Vv and RER Sv expressed per sinusoidal cells remained unchanged suggesting that the balance between the 2 hypothetical functions of the PSC, namely fibrogenesis and storage of vitamin A, was maintained. In conclusion, changes of EC and PSC after PCA result mainly in thickening of the sinusoidal barrier. This increase may impair exchanges between the sinusoidal lumen and Disse space and contribute to functional abnormalities.
大鼠门静脉分流会导致肝萎缩，并使动脉血流量增加，伴有微循环障碍。本研究的目的是使用形态计量学技术研究这些障碍对肝窦屏障（内皮细胞和窦周细胞）的影响。对行门腔静脉吻合术（PCA）的大鼠和假手术配对喂养的对照组大鼠在分流术后3个月进行研究。PCA组的窦状隙体积密度增加，但不显著，与假手术配对喂养的大鼠相比，总内皮细胞（EC）和窦周细胞（PSC）的体积密度（Vv）增加了104.54%。EC的Vv增加与表面密度（Sv）增加无关，这表明小窗孔数量减少，细胞厚度增加。这一解释支持了形态学观察结果。PSC的Vv增加主要与其内皮下突起的Vv增加有关，而不是细胞体的Vv增加。每个窦周细胞的脂质Vv和粗面内质网Sv保持不变，这表明PSC的两种假设功能，即纤维生成和维生素A储存之间的平衡得以维持。总之，PCA术后EC和PSC的变化主要导致肝窦屏障增厚。这种增厚可能会损害窦状隙腔与狄氏间隙之间的物质交换，并导致功能异常。
