Dubuisson L, Vonnahme F J, Stzark F, Bioulac-Sage P, Balabaud C
Liver. 1985 Feb;5(1):21-8. doi: 10.1111/j.1600-0676.1985.tb00011.x.
Hyperplastic focal areas were investigated in livers of male rats 1 and 9 months after portacaval anastomosis (PCA) by light and electron microscopy. These alterations, predominantly found in periportal areas, were characterized by light microscopy as clusters of enlarged hepatocytes along narrowed sinusoids, contrasting in the remaining liver acinus with smaller hepatocytes along widened sinusoids. No differences were observed between 1 and 9 months PCA except for glycogen content, which was homogeneously distributed in the liver at 1 month but completely lacking in foci at 9 months. The most striking ultrastructural alterations were the sinusoids delimited in these hyperplastic areas by a thickened barrier consisting of thick endothelial cells encircled by numerous subendothelial processes of the perisinusoidal fat-storing cells. Deep and widened recesses of the sinusoidal lumen separated the two-cell-thick plates of the hyperplastic cells. Hepatocytes in foci, thought to represent regenerative areas, tend to increase their exchange surface. Their progressive loss in glycogen and their two-cell-thick plates architecture should be in favour of a potential malignancy. However, the spontaneous evolution of these foci which do not necessarily give rise to nodules, as well as the lack of other features of transformation, do not support this possibility.
通过光学显微镜和电子显微镜对门腔静脉吻合术（PCA）后1个月和9个月的雄性大鼠肝脏中的增生性病灶区域进行了研究。这些改变主要见于门静脉周围区域，光学显微镜下其特征为沿变窄的肝血窦呈簇状增大的肝细胞，与肝腺泡其余部分沿增宽肝血窦的较小肝细胞形成对比。PCA术后1个月和9个月之间，除糖原含量外未观察到差异，糖原在1个月时在肝脏中均匀分布，但在9个月时病灶中完全缺乏。最显著的超微结构改变是这些增生区域的肝血窦由增厚的屏障界定，该屏障由增厚的内皮细胞组成，周围有许多贮脂细胞的内皮下突起环绕。肝血窦腔的深而宽的凹陷将增生细胞的两层细胞厚的板层分隔开。病灶中的肝细胞被认为代表再生区域，倾向于增加其交换表面。它们糖原的逐渐丧失及其两层细胞厚的板层结构应有利于潜在的恶性肿瘤。然而，这些病灶不一定会形成结节的自发演变，以及缺乏其他转化特征，不支持这种可能性。
