Ma Rihai, Wang Anmin, Yang Meng, Huang Zihua, Liu Guoman, Wei Qing, Lu Yuan, Wei Huamei, Wang Jianchu, Tang Qianli, Pu Jian
Department of Hepatobiliary Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi Zhuang Autonomous Region, 533000, China.
Graduate College of Youjiang Medical University for Nationalities, Guangxi Zhuang Autonomous Region, 533000, China.
Heliyon. 2024 Aug 23;10(17):e36517. doi: 10.1016/j.heliyon.2024.e36517. eCollection 2024 Sep 15.
Hepatocellular carcinoma (HCC) as the malignant cancers with high morbidity. The EMT of HCC has closely linked to the metastasis and recurrence. Moreover, tumor-associated macrophages (TAMs) can interact with HCC cells in the immune microenvironment; the M2 polarization of TAMs enhance the HCC cells EMT. The mechanism between HCC cells and TAMs is still unclear and our study was aimed to uncover it.
We performed RT-qPCR and western to detach the RNA and protein expression. The relationship among has_circ_0000092, U2AF2, SMC1A and IL24 were revealed through mechanism experiments. Rescue assays were implemented to determine how circ_0000092 modulates M2 polarization of TAMs.
As detected by RT-qPCR, has_circ_0000092 was with high expression in HCC cells and could recruit U2AF2 to promote transcription of SMC1A. Moreover, circ_0000092 could control macrophage M2 polarization via promoting IL24 expression in HCC cells.
To conclude, hsa_circ_0000092 can up-regulates IL24 by SMC1A to induce macrophages M2 polarization.
肝细胞癌（HCC）是发病率较高的恶性肿瘤。HCC的上皮-间质转化（EMT）与转移和复发密切相关。此外，肿瘤相关巨噬细胞（TAM）可在免疫微环境中与HCC细胞相互作用；TAM的M2极化增强HCC细胞的EMT。HCC细胞与TAM之间的机制仍不清楚，我们的研究旨在揭示这一机制。
我们进行了逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹法以检测RNA和蛋白质表达。通过机制实验揭示了环状RNA_0000092（has_circ_0000092）、U2AF2、SMC1A和白细胞介素24（IL24）之间的关系。进行拯救实验以确定circ_0000092如何调节TAM的M2极化。
通过RT-qPCR检测，has_circ_0000092在HCC细胞中高表达，并且可以募集U2AF2以促进SMC1A的转录。此外，circ_0000092可通过促进HCC细胞中IL24的表达来控制巨噬细胞的M2极化。
总之，hsa_circ_0000092可通过SMC1A上调IL24以诱导巨噬细胞M2极化。
