Rezaie Niloofar, Aghamohammad Shadi, Haj Agha Gholizadeh Khiavi Elham, Khatami Shohreh, Sohrabi Aria, Rohani Mahdi
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Heliyon. 2024 Aug 31;10(17):e37279. doi: 10.1016/j.heliyon.2024.e37279. eCollection 2024 Sep 15.
Maintaining a well-rounded and healthy diet is essential to promote the well-being and optimal performance of the body, especially for those suffering from Inflammatory Bowel Disease (IBD). The objective of this study is to examine whether probiotics and postbiotics can modulate oxidative stress and inflammation, and to evaluate the properties of these compounds.
A total of eighty eight strains of and were assessed for their antioxidant activities. C57BL/6 mice were allocated into four groups: normal diet (ND) + PBS, ND + DSS, ND + DSS + 10⁹ cfu/ml of probiotics, and ND + DSS + 10⁹ cfu/ml of postbiotics. Biochemical antioxidant assays, along with colitis indices, were evaluated. The ELISA assay was conducted to measure oxidant/antioxidant properties and cytokines. Additionally, the genes enrolled in NF-kB and Nrf2 signaling pathways was analyzed.
In comparison to the groups exposed to DSS alone, mice that received our native agents in addition to DSS demonstrated an improvement in the negative effects induced by DSS on DAI and pathological scores, as well as on colon length and body weight. The levels of cytokines and antioxidant markers have also been normalized following the administration of our native agents, along with molecular markers. It should also be noted that our native postbiotic was able to develop more pronounced and significant anti-inflammatory and antioxidant effects in comparison to the probiotic strains.
In this study, our native postbiotic has demonstrated a more pronounced ability to exhibit antioxidant and anti-inflammatory effects. This finding is particularly important for individuals with impaired immune function, for whom the use of live bacteria could be risky. Therefore, the utilization of agents like probiotics and postbiotics, which come with minimal side effects in compared to chemical drugs, could be essential in managing symptoms in IBD patients.
保持全面且健康的饮食对于促进身体健康和最佳机能至关重要，尤其是对于患有炎症性肠病（IBD）的患者。本研究的目的是检验益生菌和后生元是否能调节氧化应激和炎症，并评估这些化合物的特性。
总共评估了88株[具体菌名未给出]的抗氧化活性。将C57BL/6小鼠分为四组：正常饮食（ND）+磷酸盐缓冲液（PBS）、ND+葡聚糖硫酸钠（DSS）、ND+DSS+10⁹ cfu/ml益生菌、ND+DSS+10⁹ cfu/ml后生元。评估了生化抗氧化测定以及结肠炎指标。进行酶联免疫吸附测定（ELISA）以测量氧化/抗氧化特性和细胞因子。此外，分析了参与核因子-κB（NF-κB）和核因子E2相关因子2（Nrf2）信号通路的基因。
与仅暴露于DSS的组相比，除DSS外还接受我们的天然制剂的小鼠在DSS对疾病活动指数（DAI）和病理评分以及结肠长度和体重所诱导的负面影响方面有改善。在给予我们的天然制剂后，细胞因子和抗氧化标志物的水平以及分子标志物也恢复正常。还应注意的是，与益生菌菌株相比，我们的天然后生元能够产生更显著的抗炎和抗氧化作用。
在本研究中，我们的天然后生元已证明具有更显著的抗氧化和抗炎作用。这一发现对于免疫功能受损的个体尤为重要，因为使用活细菌可能有风险。因此，与化学药物相比副作用最小的益生菌和后生元等制剂的使用，对于管理IBD患者的症状可能至关重要。
