Kovarikova Simona, Blahova Jana, Vanova Ivana, Marsalek Petr
Department of Animal Welfare and Protection and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic.
Department of Physiology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic.
Vet Med (Praha). 2024 Aug 29;69(8):273-280. doi: 10.17221/30/2024-VETMED. eCollection 2024 Aug.
Determination of the urinary protein-to-creatinine ratio (UPC) is an important tool in the quantification of proteinuria in animals. However, the result may be affected by the different methods of determining the urinary protein concentration. The aim of this study was to compare the turbidimetric method using benzethonium chloride and the colorimetric method using pyrogallol red in the measurement of the urinary protein concentration in dogs, cats, guinea pigs and horses. A total of 464, 192, 216 and 119 urine samples from dogs, cats, guinea pigs and horses were examined in the study, respectively. The group consisted of animals of both sexes and different ages, and, in the dogs and cats, it included both healthy animals and those with various health problems. In the group of horses and guinea pigs, only clinically healthy animals were included. A total of 347, 185, 103 and 100 samples from the dogs, cats, guinea pigs and horses were used in the statistical analysis; the other values were excluded as they were below the detection limit. According to the Passing-Bablok analysis, there was a significant constant and proportional difference in the horses. In the dogs, cats and guinea pigs, there was a significant constant difference, but no proportional difference. The Bland-Altman method showed significant bias between the two methods in the horses and cats, but not in the dogs and guinea pigs. In the dogs and cats, the agreement between the two methods was tested and expressed as Cohen's kappa (κ). In the cats, it was almost perfect for the proteinuric samples (κ = 0.823 3) and significant for the non-proteinuric samples (κ = 0.804 9). In the dogs, the agreement was significant for the non-proteinuric samples (κ = 0.621 5) and only moderate for the proteinuric samples (κ = 0.527 5). The influence of the method used to determine the urinary protein concentration should be taken into account when evaluating the UPC. Repeated examinations in one patient should be performed with the same method.
测定尿蛋白与肌酐比值（UPC）是量化动物蛋白尿的一项重要手段。然而，该结果可能会受到不同尿蛋白浓度测定方法的影响。本研究的目的是比较在犬、猫、豚鼠和马的尿蛋白浓度测定中，使用苄索氯铵的比浊法和使用邻苯三酚红的比色法。该研究分别检测了来自犬、猫、豚鼠和马的464、192、216和119份尿液样本。该组动物包括不同性别和年龄的个体，在犬和猫中，既有健康动物，也有患有各种健康问题的动物。在马和豚鼠组中，仅纳入临床健康的动物。来自犬、猫、豚鼠和马的347、185、103和100份样本用于统计分析；其他数值因低于检测限而被排除。根据帕辛-巴布洛克分析，马中存在显著的常数和比例差异。在犬、猫和豚鼠中，存在显著的常数差异，但无比例差异。布兰德-奥特曼法显示，两种方法在马和猫中存在显著偏差，但在犬和豚鼠中不存在。在犬和猫中，对两种方法之间的一致性进行了检验，并以科恩kappa（κ）表示。在猫中，蛋白尿样本的一致性几乎完美（κ = 0.823 3），非蛋白尿样本的一致性显著（κ = 0.804 9）。在犬中，非蛋白尿样本的一致性显著（κ = 0.621 5），蛋白尿样本的一致性仅为中等（κ = 0.527 5）。在评估UPC时，应考虑用于测定尿蛋白浓度的方法的影响。对同一患者的重复检查应使用相同的方法进行。
