铁在铁蛋白和含铁血黄素介导的脂质体脂质过氧化中的作用。
The role of iron in ferritin- and haemosiderin-mediated lipid peroxidation in liposomes.
作者信息
O'Connell M J, Ward R J, Baum H, Peters T J
出版信息
Biochem J. 1985 Jul 1;229(1):135-9. doi: 10.1042/bj2290135.
Abstract
Ferritin and haemosiderin were shown, by the measurement of malondialdehyde production and loss of polyunsaturated fatty acids, to stimulate lipid peroxidation in liposomes. At pH 7.4 ascorbate was additionally required to achieve peroxidation; however, peroxidation occurred at pH 4.5 in the presence of iron-proteins alone. The damage was completely inhibited by the incorporation of chain-breaking antioxidants (alpha-tocopherol and butylated hydroxytoluene) into the liposomes. Metal chelators (desferrioxamine and EDTA) also completely inhibited lipid peroxidation. These and further results indicate that, at pH 4.5, even in the absence of a reducing agent, iron is released from haemosiderin and can mediate oxidative damage to a lipid membrane.
摘要
通过测定丙二醛生成量和多不饱和脂肪酸损失量发现，铁蛋白和含铁血黄素可刺激脂质体中的脂质过氧化反应。在pH 7.4时，还需要抗坏血酸才能引发过氧化反应；然而，在仅存在铁蛋白的情况下，pH 4.5时就会发生过氧化反应。通过向脂质体中加入链断裂抗氧化剂（α-生育酚和丁基化羟基甲苯），损伤被完全抑制。金属螯合剂（去铁胺和乙二胺四乙酸）也能完全抑制脂质过氧化。这些及其他结果表明，在pH 4.5时，即使没有还原剂，铁也会从含铁血黄素中释放出来，并介导对脂质膜的氧化损伤。
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