Department of Pharmacy, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Bangladesh Pharmacogenomics Research Network (BdPGRN), Dhaka, 1229, Bangladesh.
Reprod Sci. 2024 Nov;31(11):3420-3427. doi: 10.1007/s43032-024-01679-5. Epub 2024 Sep 19.
Cervical cancer (CC) has been the prominent cause of cancer-associated fatalities among women in developing countries. In terms of occurrence and mortality, it is ranked second in Bangladesh. Although different genetic polymorphisms linked with this cancer have been investigated over time, the association between the HOTAIR rs7958904 variant and cervical cancer is being reported for the first time in Bangladeshi women. RT-PCR-based TaqMan assay was employed to perform this case-control study on 200 cervical cancer patients and 148 healthy volunteers. Both cases and controls had average ages of 57.5 and 52.5 years, respectively. According to Hardy-Weinberg equilibrium, the rs7958904 allele of HOTAIR gene pretended no deviation for both cases and control groups. The genotyping results showed that rs7958904 has a significant correlation to the development of cervical cancer in different genetic association models, such as co-dominant 1 (CC vs. GG: OR = 1.67, p = 0.0435), co-dominant 2 (CC vs. GG: OR = 3.13, p = 0.0006), co-dominant 3 (CC vs. CG: OR = 1.88, p = 0.0384), dominant (CG + CC vs. GG: OR = 1.98, p = 0.004), recessive (CC vs. GG + CG: OR = 2.25, p = 0.005), and allele model (C vs. G: OR = 1.70, p = 0.0006). In conclusion, the HOTAIR rs7958904 variant has a substantial role in cervical cancer development in Bangladeshi women. Further functional studies with a larger population size are required to support our findings.
宫颈癌（CC）一直是发展中国家女性癌症相关死亡的主要原因。就发病率和死亡率而言，它在孟加拉国排名第二。尽管随着时间的推移，已经研究了与这种癌症相关的不同遗传多态性，但 HOTAIR rs7958904 变体与宫颈癌之间的关联在孟加拉国女性中是首次报道。本病例对照研究采用基于 RT-PCR 的 TaqMan 检测法，对 200 名宫颈癌患者和 148 名健康志愿者进行了研究。病例组和对照组的平均年龄分别为 57.5 岁和 52.5 岁。根据 Hardy-Weinberg 平衡，HOTAIR 基因的 rs7958904 等位基因在病例组和对照组中均无偏差。基因分型结果表明，rs7958904 在不同的遗传关联模型中与宫颈癌的发生具有显著相关性，如共显性 1（CC 与 GG：OR=1.67，p=0.0435）、共显性 2（CC 与 GG：OR=3.13，p=0.0006）、共显性 3（CC 与 CG：OR=1.88，p=0.0384）、显性（CG+CC 与 GG：OR=1.98，p=0.004）、隐性（CC 与 GG+CG：OR=2.25，p=0.005）和等位基因模型（C 与 G：OR=1.70，p=0.0006）。总之，HOTAIR rs7958904 变体在孟加拉国女性宫颈癌的发生中起着重要作用。需要进一步进行功能研究，以更大的人群规模来支持我们的发现。
