Yang Ruiguo, Broussard Joshua A, Green Kathleen J, Espinosa Horacio D
Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, United States.
Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE 68588, United States.
Extreme Mech Lett. 2018 Apr;20:125-139. doi: 10.1016/j.eml.2017.12.002. Epub 2017 Dec 7.
Cell-cell adhesions maintain the mechanical integrity of multicellular tissues and have recently been found to act as mechanotransducers, translating mechanical cues into biochemical signals. Mechanotransduction studies have primarily focused on focal adhesions, sites of cell-substrate attachment. These studies leverage technical advances in devices and systems interfacing with living cells through cell-extracellular matrix adhesions. As reports of aberrant signal transduction originating from mutations in cell-cell adhesion molecules are being increasingly associated with disease states, growing attention is being paid to this intercellular signaling hub. Along with this renewed focus, new requirements arise for the interrogation and stimulation of cell-cell adhesive junctions. This review covers established experimental techniques for stimulation and interrogation of cell-cell adhesion from cell pairs to monolayers.
细胞间黏附维持多细胞组织的机械完整性，最近发现其可作为机械转导器，将机械信号转化为生化信号。机械转导研究主要集中在粘着斑，即细胞与底物附着的位点。这些研究利用了通过细胞-细胞外基质黏附与活细胞连接的设备和系统的技术进步。由于越来越多的报道表明，源自细胞间黏附分子突变的异常信号转导与疾病状态相关，因此这个细胞间信号枢纽受到了越来越多的关注。随着这一重新关注，对细胞间黏附连接的检测和刺激也出现了新的要求。本综述涵盖了从细胞对到单层细胞间黏附的刺激和检测的既定实验技术。
