van Ulsen J, van Zuuren-van der Horst A, Tjiam K H, van der Heijst Y, Michel M F, van Eijk R V, van Joost T, Stolz E
Eur J Clin Microbiol. 1985 Aug;4(4):397-9. doi: 10.1007/BF02148691.
The prototype of a solid-phase enzyme immunoassay for the detection of Chlamydia trachomatis antigen was tested in 403 men and 135 women attending a venereal disease clinic. Culture on HeLa 229 cells was used as reference method. In men the overall sensitivity and specificity of the enzyme immunoassay was 70% and 98.5% respectively. In the 158 men with non-gonococcal urethritis the sensitivity and specificity was 73% and 96.5% respectively, in the 31 men with post-gonococcal urethritis 82% and 100%, in the 48 men with gonococcal urethritis 67% and 100%, in the 125 men with discharge but normal urinary sediment 54% and 99.1% and in the 41 men with no signs or symptoms 50% and 100%. In women the sensitivity and specificity of the assay was 70% and 92% respectively. On account of the low degree of sensitivity in both men and women and the low degree of specificity in women the prototype of the enzyme immunoassay is not yet considered suitable for clinical use. An improved model is being developed.
一种用于检测沙眼衣原体抗原的固相酶免疫测定法原型，在一家性病诊所就诊的403名男性和135名女性中进行了测试。以HeLa 229细胞培养作为参考方法。在男性中，酶免疫测定法的总体敏感性和特异性分别为70%和98.5%。在158名患有非淋菌性尿道炎的男性中，敏感性和特异性分别为73%和96.5%；在31名患有淋菌性尿道炎后尿道炎的男性中，分别为82%和100%；在48名患有淋菌性尿道炎的男性中，分别为67%和100%；在125名有分泌物但尿沉渣正常的男性中，分别为54%和99.1%；在41名无体征或症状的男性中，分别为50%和100%。在女性中，该测定法的敏感性和特异性分别为70%和92%。由于该酶免疫测定法在男性和女性中的敏感性较低，在女性中的特异性也较低，因此该酶免疫测定法原型尚未被认为适合临床使用。一种改进的模型正在开发中。
