白杨素 A-G：深海来源放线菌 228DD-066 的羟基化脂肪胺衍生物及其细胞毒性活性。

Albusamides A-G: Hydroxylated Fatty Amine Derivatives from the Deep-Sea-Derived Actinomycete 228DD-066 and Their Cytotoxic Activity.

机构信息

Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology, 385 Haeyang-ro, Yeoungdo-gu, Busan 49111, Republic of Korea.

Department of Marine Biotechnology, University of Science and Technology (UST), 217 Gajungro, Yuseong-gu, Daejeon 34113, Republic of Korea.

出版信息

J Nat Prod. 2024 Oct 25;87(10):2432-2440. doi: 10.1021/acs.jnatprod.4c00683. Epub 2024 Sep 21.

DOI:10.1021/acs.jnatprod.4c00683

PMID:39305259

Abstract

A series of new hydroxylated fatty amine derivatives, albusamides A-G (-), along with four known compounds (-), which are reported for the first time from a natural source, were isolated from the culture broth of 228DD-066 derived from a deep-sea sediment sample gathered off the coast of Dokdo Island, Republic of Korea. Their structures were elucidated through the comprehensive analysis of 1D and 2D NMR spectra and HRESIMS, and absolute configurations were determined using the modified Mosher's method. Biological evaluations against solid and blood cancer cell lines revealed that these new metabolites have moderate to strong cytotoxic activity. Compound exhibited high cytotoxic activity with GI values ranging from 0.4 to 0.6 μM against solid cancer cell lines and exhibited the strongest cytotoxicity (GI value = 0.2 μM) against the WSU-DLCL2 blood cancer cell line.

摘要

从韩国独岛海域深海沉积物样本中分离得到的 228DD-066 菌株发酵液中，发现了一系列新的羟基化脂肪胺衍生物（albusamides A-G，-）以及 4 种已知化合物（-），这是它们首次在天然产物中被发现。通过对 1D 和 2D NMR 谱和 HRESIMS 的综合分析，确定了它们的结构，并使用改良的 Mosher 法确定了它们的绝对构型。对固体和血液癌细胞系的生物评价表明，这些新代谢产物具有中等至较强的细胞毒性。化合物对固体癌细胞系表现出较高的细胞毒性，GI 值范围为 0.4 至 0.6 μM，对 WSU-DLCL2 血液癌细胞系表现出最强的细胞毒性（GI 值=0.2 μM）。