Dos Santos Teles Oliveira Linda Kimberlin, da Silva Michelli Santos, Munis Tivian Raphaella Melo, Valença Gabriel Moreira, de França Kamila Pereira, Alves Janaina Danielle, Júnior Antônio Marques Pereira, da Silva Priscilla Elias Ferreira, Medeiros Jansen Fernandes
Laboratório de Entomologia, Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho, RO, Brasil; Programa de Pós-graduação em Biologia Experimental - PGBIOEXP, Fiocruz Rondônia/ UNIR, Porto Velho, RO, Brasil.
Laboratório de Entomologia, Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho, RO, Brasil.
Acta Trop. 2024 Dec;260:107407. doi: 10.1016/j.actatropica.2024.107407. Epub 2024 Sep 19.
This study aimed to assess the diversity patterns of sand fly fauna across different strata and detect Leishmania DNA in these insects in the Mapinguari National Park in Rondônia and Amazonas states, Northern Brazil. Sand flies were collected with "HP" light traps in the canopy (15 m) and at ground level (1 m) on two trails, during August and November 2021 and March and August 2022. Polymerase chain reaction and DNA sequencing were used to identify Leishmania species. A total of 8,040 individuals (2,303♂ - 28.64 %, 5,737♀ - 71.36 %) were collected and 53 species and 13 genera were identified. The most abundant species were Psychodopygus chagasi (21.00 %), Trichophoromyia ubiquitalis (13.61 %) Psychodopygus davisi (11.69 %), Nyssomyia fraihai (10.58 %), Nyssomyia antunesi (6.68 %) and Nyssomyia richardwardi (5.55 %). Species diversity was greater in the canopy (Shannon index H' = 10.8 common species) when compared to ground level (H' = 10.5 common species). We observed a minimum infection rate of 0.45 % (22/4,868 females), in which Leishmania braziliensis DNA was found in Ps. chagasi and Le. lainsoni DNA in Ps. chagasi, Ny. richardwardi, Ps. ayrozai and Th. ubiquitalis. The minimum infection rate of Leishmania in the canopy was 0.47 % (19/4,031) and in the ground was 0.52 % (3/567). In the present study, we observed Le. lainsoni DNA in females of Ny. richardwardi for the first time. The data presented in this study contribute to understanding sand fly diversity and its distribution between the states of Rondônia and Amazonas. They may be useful for implementing targeted control measures to reduce the spread of leishmaniasis and implement entomological surveillance strategies.
本研究旨在评估巴西北部朗多尼亚州和亚马孙州马平瓜里国家公园不同地层中白蛉动物群的多样性模式，并检测这些昆虫体内的利什曼原虫DNA。2021年8月和11月以及2022年3月和8月期间，在两条小径的树冠层（15米）和地面（1米）用“HP”诱虫灯收集白蛉。采用聚合酶链反应和DNA测序鉴定利什曼原虫种类。共收集到8040只个体（雄性2303只 - 占28.64%，雌性5737只 - 占71.36%），鉴定出53个物种和13个属。最丰富的物种是查加斯白蛉（Psychodopygus chagasi，占21.00%）、泛在毛蠓（Trichophoromyia ubiquitalis，占13.61%）、戴维斯白蛉（Psychodopygus davisi，占11.69%）、弗氏奈氏白蛉（Nyssomyia fraihai，占10.58%）、安图内斯奈氏白蛉（Nyssomyia antunesi，占6.68%）和理查德沃迪奈氏白蛉（Nyssomyia richardwardi，占5.55%）。与地面（香农指数H' = 10.5个常见物种）相比，树冠层的物种多样性更高（香农指数H' = 10.8个常见物种）。我们观察到最低感染率为0.45%（22/4868只雌性），其中在查加斯白蛉中发现了巴西利什曼原虫DNA，在查加斯白蛉、理查德沃迪奈氏白蛉、阿约扎伊白蛉（Ps. ayrozai）和泛在毛蠓中发现了赖氏利什曼原虫（Le. lainsoni）DNA。树冠层利什曼原虫的最低感染率为0.47%（19/4031），地面为0.52%（3/567）。在本研究中，我们首次在理查德沃迪奈氏白蛉雌性中发现了赖氏利什曼原虫DNA。本研究提供的数据有助于了解朗多尼亚州和亚马孙州白蛉的多样性及其分布。它们可能有助于实施有针对性的控制措施，以减少利什曼病的传播并实施昆虫学监测策略。
