Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan.
School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
J Food Drug Anal. 2018 Jul;26(3):1105-1112. doi: 10.1016/j.jfda.2018.01.001. Epub 2018 Jan 17.
Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.
枸杞，茄科药物，广泛用作功能性食品和中药。从中国不同地区采集的样本在化学成分上并不完全相同，这可能会影响其生物活性。虽然已经报道了许多色谱和光谱方法来测定甜菜碱和其他生物活性氨基酸的浓度，但由于其他具有低紫外吸收和昂贵质谱设备的极性物质的干扰，这些技术的适用性降低。在本研究中，成功开发了一种基于强阳离子交换固相萃取程序和 1H NMR 的快速可靠方法，可同时测定各种枸杞中的甜菜碱、柠檬酸、苏氨酸、丙氨酸和脯氨酸。此外，还应用了基于 PULCON 原理的 ERETIC 2 方法，并与常规方法进行了比较。这种可行且实用的方法为控制来自不同来源的商业枸杞的质量提供了非常有力的工具。
