Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology Bannu, Khyber Pakhtunkhwa, Pakistan.
BMC Complement Altern Med. 2012 Jul 9;12:90. doi: 10.1186/1472-6882-12-90.
Sonchus asper (SAME) is used as a folk medicine in hepatic disorders. In this study, the hepatoprotective effects of the methanol extract of SAME was evaluated against carbon tetrachloride (CCl4)-induced liver injuries in rats.
To evaluate the hepatoprotective effects of SAME, 36 male Sprague-Dawley rats were equally divided into 6 groups. Rats of Group I (control) were given free access to approved feed and water. Rats of Group II were injected intraperitoneally with CCl4 (3 ml/kg) as a 30% solution in olive oil (v/v) twice a week for 4 weeks. Animals of Groups III (100 mg/kg) and IV (200 mg/kg) received SAME, whereas those of Group V were given silymarin via gavage (100 mg/kg) after 48 h of CCl4 treatment. Group VI received SAME (200 mg/kg) twice a week for 4 weeks without CCl4 treatment. Various parameters, such as the serum enzyme levels, serum biochemical marker levels, antioxidant enzyme activities, and liver histopathology were used to estimate the hepatoprotective efficacy of SAME.
The administration of SAME and silymarin significantly lowered the CCl4-induced serum levels of hepatic marker enzymes (aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase), cholesterol, low-density lipoprotein, and triglycerides while elevating high-density lipoprotein levels. The hepatic contents of glutathione and activities of catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, and glutathione reductase were reduced. The levels of thiobarbituric acid-reactive substances that were increased by CCl4 were brought back to control levels by the administration of SAME and silymarin. Liver histopathology showed that SAME reduced the incidence of hepatic lesions induced by CCl4 in rats.
SAME may protect the liver against CCl4-induced oxidative damage in rats.
苦苣菜（SAME）作为一种民间药物，用于治疗肝脏疾病。本研究评价了甲醇提取物 SAME 对四氯化碳（CCl4）诱导的大鼠肝损伤的保护作用。
为了评价 SAME 的保肝作用，将 36 只雄性 Sprague-Dawley 大鼠等分为 6 组。第 I 组（对照组）大鼠自由摄入批准的饲料和水。第 II 组大鼠每周两次腹膜内注射 CCl4（3 ml/kg），溶于橄榄油（v/v）中，共 4 周。第 III 组（100 mg/kg）和第 IV 组（200 mg/kg）给予 SAME，而第 V 组在 CCl4 处理后 48 h 给予水飞蓟宾灌胃（100 mg/kg）。第 VI 组每周两次给予 SAME（200 mg/kg），共 4 周，不给予 CCl4。使用血清酶水平、血清生化标志物水平、抗氧化酶活性和肝组织病理学等参数来评估 SAME 的保肝效果。
SAME 和水飞蓟宾的给药显著降低了 CCl4 诱导的血清肝标志物酶（天冬氨酸氨基转移酶、丙氨酸氨基转移酶和乳酸脱氢酶）、胆固醇、低密度脂蛋白和三酰甘油的水平，同时提高了高密度脂蛋白的水平。肝组织中的谷胱甘肽含量以及过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽 S-转移酶和谷胱甘肽还原酶的活性降低。CCl4 引起的硫代巴比妥酸反应物质水平升高，经 SAME 和水飞蓟宾给药后恢复至对照水平。肝组织病理学显示，SAME 降低了 CCl4 诱导的大鼠肝损伤的发生率。
SAME 可能对 CCl4 诱导的大鼠肝氧化损伤具有保护作用。
