Hanssen L E, Osnes M, Myren J
Scand J Gastroenterol. 1978;13(3):325-30. doi: 10.3109/00365527809179828.
The main pancreatic duct was cannulated in 12 individuals with a teflon catheter by means of a side-viewing duodenscope. Six individuals received a duodenal infusion of 40 ml 100 mmol/l HCl over 5 min, while the other six served as controls for basal pancreatic secretion. Pancreatic juice was collected in 5-min samples, and blood was frequently drawn for radioimmunoassay of immunoreactive secretin (IRS). In the control group, during 20-min cannulation of the main pancreatic duct, no effect was seen on basal secretion of water, bicarbonate, or alpha-amylase--nor did the IRS levels change. After duodenal acidification there was a significant increase in IRS (p less than 0.02), reaching the highest level at 7 min. The mean flow rate, bicarbonate concentration, and bicarbonate output showed a significant increase as compared to the control group (p less than 0.02), the highest levels being reached in the third 5-min period after the start of the duodenal acidification. The alpha-amylase output was also significantly higher after acidification (p less than 0.02) than in the control group, but the mean alpha-amylase concentration decreased after acidification, reaching its nadir in the third 5-min sample (p less than 0.02). The present results demonstrate a basal and HCl-stimulated pancreatic secretion collected by endoscopic cannulation of the main pancreatic duct in man together with plasma IRS levels.
采用侧视十二指肠镜，用聚四氟乙烯导管对12名受试者的主胰管进行插管。6名受试者在5分钟内接受40毫升100毫摩尔/升盐酸的十二指肠灌注，另外6名作为基础胰腺分泌的对照。每隔5分钟收集一次胰液样本，并频繁采集血液用于免疫反应性促胰液素（IRS）的放射免疫测定。在对照组中，主胰管插管20分钟期间，基础水、碳酸氢盐或α淀粉酶分泌未见变化，IRS水平也未改变。十二指肠酸化后，IRS显著升高（p<0.02），在7分钟时达到最高水平。与对照组相比，平均流速、碳酸氢盐浓度和碳酸氢盐输出量显著增加（p<0.02），在十二指肠酸化开始后的第三个5分钟时段达到最高水平。酸化后α淀粉酶输出量也显著高于对照组（p<0.02），但酸化后平均α淀粉酶浓度降低，在第三个5分钟样本中降至最低点（p<0.02）。目前的结果表明，通过内镜插管收集人主胰管的基础和盐酸刺激的胰腺分泌以及血浆IRS水平。
