Otsuki M, Sakamoto C, Ohki A, Yuu H, Maeda M, Baba S
Dig Dis Sci. 1981 Jun;26(6):538-44. doi: 10.1007/BF01308104.
Portal plasma immunoreactive secretin (IRS) concentrations, pancreatic juice flow, and amylase output were simultaneously measured in response to intraduodenal infusion of 1-phenyl-1-hydroxy-n-pentane (PHP), as well as infusion of hydrochloric acid (HCl). These data were compared with those obtained from intravenous bolus injections of synthetic porcine secretin in anesthetized rats. The intraduodenal infusion of PHP or HCl at a rate of 2 ml/min for 2 min produced a dose-related increase in portal plasma secretin concentrations, pancreatic juice flow, and amylase output. However, the mechanism of secretin release by PHP seems to differ from that of HCl. The secretin response to 0.1 N HCl infused at a rate of 0.1 ml/min for 30 min was complete after 10 min, despite continued infusion, while PHP stimulated a secretin release which persisted for 10 min after cessation of infusion. The pH in the second portion of the duodenum, following PHP infusion, remained consistently greater than 6.3. PHP-stimulated pancreatic exocrine secretions were only partially suppressed by somatostatin, while secretin release was almost completely inhibited. However, intraduodenal PHP may stimulate the release of secretin along with other gastrointestinal hormones, and the endogenous release of these hormones may not be inhibited by somatostatin.
在麻醉大鼠中，通过十二指肠内输注1-苯基-1-羟基正戊烷（PHP）以及输注盐酸（HCl），同时测量门静脉血浆免疫反应性促胰液素（IRS）浓度、胰液流量和淀粉酶分泌量。将这些数据与通过静脉推注合成猪促胰液素获得的数据进行比较。以2 ml/min的速率十二指肠内输注PHP或HCl 2分钟，会使门静脉血浆促胰液素浓度、胰液流量和淀粉酶分泌量呈剂量相关增加。然而，PHP释放促胰液素的机制似乎与HCl不同。以0.1 ml/min的速率输注0.1 N HCl 30分钟，尽管持续输注，但促胰液素反应在10分钟后就完成了，而PHP刺激的促胰液素释放则在输注停止后持续10分钟。输注PHP后，十二指肠第二段的pH值始终保持大于6.3。PHP刺激的胰腺外分泌仅被生长抑素部分抑制，而促胰液素释放几乎被完全抑制。然而，十二指肠内的PHP可能会与其他胃肠激素一起刺激促胰液素的释放，并且这些激素的内源性释放可能不会被生长抑素抑制。
