Osnes M, Hanssen L E
Scand J Gastroenterol. 1980;15(8):1041-7. doi: 10.3109/00365528009181810.
The exocrine pancreatic secretion of water, bicarbonate, amylase, and protein and the plasma levels of immunoreactive secretin (IRS) were studied after intraduodenal infusions of bile and pancreatic juice. Pancreatic secretion was obtained by endoscopic cannulation of the main pancreatic duct. Bile and pancreatic juice were infused into the duodenum through separate catheters attached to the outside of the duodenoscope. The unstimulated secretion was collected for 20 min. After intraduodenal stimulation of the pancreatic secretion with a nearly neutral solution of dried cattle bile, juice was collected for another 20-min period. Then, pure pancreatic juice was infused into the duodenum. It was shown that pancreatic juice reduced the flow rate and output of bicarbonate, amylase, and protein significantly (p less than 0.05). A significant reduction in plasma concentration of IRS (p less than 0.05) was also found. In the controls, i.e., when no pancreatic juice was instilled into the duodenum, a further increase in flow rate, bicarbonate secretion, and IRS was found. It is concluded that the exocrine pancreatic secretion and IRS release induced by intraduodenal administration of bile may be depressed by reinfusions of pancreatic juice. The corresponding effect on bicarbonate secretion and IRS release found in this study supports the view that secretin may play an important role in the exocrine pancreatic secretion induced by intraduodenal infusion of bile.
在十二指肠内输注胆汁和胰液后，研究了胰腺外分泌的水、碳酸氢盐、淀粉酶和蛋白质以及免疫反应性促胰液素（IRS）的血浆水平。通过内镜插管主胰管获取胰液分泌。胆汁和胰液通过连接在十二指肠镜外部的单独导管注入十二指肠。收集20分钟的基础分泌液。在用近乎中性的干牛胆汁溶液对胰腺分泌进行十二指肠内刺激后，再收集20分钟的分泌液。然后，将纯胰液注入十二指肠。结果显示，胰液显著降低了碳酸氢盐、淀粉酶和蛋白质的流速及分泌量（p<0.05）。还发现血浆中IRS浓度显著降低（p<0.05）。在对照组中，即未向十二指肠内注入胰液时，发现流速、碳酸氢盐分泌和IRS进一步增加。得出结论，十二指肠内注入胆汁诱导的胰腺外分泌和IRS释放可能会因再次注入胰液而受到抑制。本研究中发现的对碳酸氢盐分泌和IRS释放的相应影响支持了促胰液素可能在十二指肠内注入胆汁诱导的胰腺外分泌中起重要作用的观点。
