Key Laboratory of Textile Fiber and Products (Wuhan Textile University), Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials &Application, Wuhan Textile University, Wuhan 430200, China.
Key Laboratory of Textile Fiber and Products (Wuhan Textile University), Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials &Application, Wuhan Textile University, Wuhan 430200, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Feb 5;326:125189. doi: 10.1016/j.saa.2024.125189. Epub 2024 Sep 22.
A method for the specific detection of glutathione (GSH) and ascorbic acid (AA) using 3-aminophenylboronic acid functionalized molybdenum disulfide quantum dots (APBA-MoS QDs) was reported. The APBA-MoS QDs synthesized using the hydrothermal method showed the strongest fluorescence intensity at an emission wavelength of 375 nm with the optimum excitation wavelength of 320 nm. The detection method was constructed by the combination of the dynamic and static quenching and the inner filter effect (IFE) of APBA-MoS QDs by permanganate (MnO) and the rapid redox reaction (1 min) of MnO by GSH or AA. The concentrations of GSH and AA showed good linear ranges of 10-500 μM and 10-100 μM, respectively, with limits of detection (LOD) of 0.476 μM and 0.185 μM, respectively. The spiked recovery tests in human serum and fresh fruit samples showed that APBA-MoS QDs had significant potential in the construction of fluorescent biological detection methods.
本文报道了一种利用 3-氨基苯硼酸功能化二硫化钼量子点（APBA-MoS QDs）特异性检测谷胱甘肽（GSH）和抗坏血酸（AA）的方法。采用水热法合成的 APBA-MoS QDs 在发射波长为 375nm 时表现出最强的荧光强度，最佳激发波长为 320nm。该检测方法是通过锰酸钾（MnO）与 APBA-MoS QDs 的动态和静态猝灭以及内滤效应（IFE）的结合以及 MnO 与 GSH 或 AA 的快速氧化还原反应（1min）构建的。GSH 和 AA 的浓度分别在 10-500μM 和 10-100μM 范围内呈现良好的线性关系，检测限（LOD）分别为 0.476μM 和 0.185μM。在人血清和新鲜水果样品中的加标回收试验表明，APBA-MoS QDs 在构建荧光生物检测方法方面具有重要的应用潜力。
