Sun Depeng, Mu Baide, Liu Yujia, Zhao Changcheng, Li Hongmei, Wang Juan, Li Tingyu, Li Guanhao, Piao Chunxiang
College of Agriculture, Yanbian University, Yanji 133002, China.
Key Innovation Laboratory for Deep and Intensive Processing of Yanbian High Quality Beef, Ministry of Agriculture and Rural Affairs, Yanji 133002, China.
Foods. 2024 Sep 11;13(18):2879. doi: 10.3390/foods13182879.
Dry-aging is a postmortem process that can substantially enhance the texture and flavour of beef. This study entailed suspending Yanbian cattle M. gluteus medius in the aging cabinet, maintained at a temperature of 2-4 °C and a relative humidity of 85 ± 5% for 35 days. Throughout this period, samples were systematically collected every 7 days. The widely targeted metabolomic analysis has been used in this investigation to analyse the dynamic changes in Yanbian cattle metabolites during dry-aging. A total of 883 metabolites were identified, with amino acids and their metabolites representing the largest proportion. Multivariate statistical analysis showed that 373 metabolites were identified as differential metabolites that changed significantly during the dry-aging process, including metabolites of amino acids, glycerophospholipids, and nucleotides and their metabolites. Additionally, 308 metabolites exhibited various increasing trends with time in dry-aging. The analysis of KEGG pathway analysis showed that ABC transporters, glycerophospholipid, and arachidonic acid metabolism are the most important metabolic pathways during dry-aging. These findings can guide technological developments in the meat processing sector and provide valuable insights into the metabolic traits and pathways of Yanbian cattle during the dry-aging process.
干式熟成是一种宰后处理过程，可显著提升牛肉的质地和风味。本研究将延边黄牛的臀中肌悬挂于熟成柜中，在温度为2-4°C、相对湿度为85±5%的条件下放置35天。在此期间，每隔7天系统地采集样本。本研究采用广泛靶向代谢组学分析方法，分析延边黄牛在干式熟成过程中代谢物的动态变化。共鉴定出883种代谢物，其中氨基酸及其代谢物占比最大。多变量统计分析表明，有373种代谢物被鉴定为在干式熟成过程中发生显著变化的差异代谢物，包括氨基酸、甘油磷脂、核苷酸及其代谢物的代谢物。此外，308种代谢物在干式熟成过程中随时间呈现出不同的增加趋势。KEGG通路分析表明，ABC转运蛋白、甘油磷脂和花生四烯酸代谢是干式熟成过程中最重要的代谢途径。这些发现可为肉类加工行业的技术发展提供指导，并为延边黄牛在干式熟成过程中的代谢特征和途径提供有价值的见解。
