Lu Shi, Wang Qi, Yin Junqi, Zheng Shubo, Gao Tingting, Zhou Xudong, Zhang Jianxin, Xing Yuexian, Ma Yingjie, Wang Min, Zhou Delong, Lu Ming, Liu Wenguo, Wang Piwu, Zhang Zhijun
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China.
Jilin Jinong Hi-tech Inc., Ltd., Kemao Street, No.303, Gongzhuling 136100, China.
Life (Basel). 2024 Aug 23;14(9):1057. doi: 10.3390/life14091057.
Leaf width is a key determinant of planting density and photosynthetic efficiency. In an effort to determine which genes regulate maize plant leaf width, we performed a genome-wide association study (GWAS) of 1.49 × 106 single nucleotide polymorphisms (SNPs) in 80 sequenced backbone inbred maize lines in Jilin Province, China, based upon phenotypic leaf width data from two years. In total, 14 SNPs were identified as being significantly related to leaf width ( < 0.000001), with these SNPs being located on chromosomes 1, 2, 3, 5, 6, 7, 8, and 9. A total of five candidate genes were identified within a mean linkage disequilibrium (LD) distance of 9.7 kb, with a significant SNP being identified within the candidate gene. RNA was then isolated from 12 different inbred maize lines from this GWAS study cohort and was used to conduct qPCR analyses which revealed significant differences in expression among strains exhibiting significant differences in leaf width. Based on an assessment of EMS mutant lines harboring a conserved amino acid stop mutation and two non-synonymous mutations in that exhibited a narrow leaf width, these data suggested that is a key regulator of maize leaf width.
叶宽是种植密度和光合效率的关键决定因素。为了确定哪些基因调控玉米植株的叶宽，我们基于来自中国吉林省80个测序骨干自交系玉米两年的叶宽表型数据，对149万个单核苷酸多态性（SNP）进行了全基因组关联研究（GWAS）。总共鉴定出14个与叶宽显著相关的SNP（P<0.000001），这些SNP位于第1、2、3、5、6、7、8和9号染色体上。在平均9.7kb的连锁不平衡（LD）距离内共鉴定出5个候选基因，并且在候选基因内鉴定出一个显著的SNP。然后从该GWAS研究群体的12个不同自交系玉米中分离RNA，并用于进行qPCR分析，结果显示叶宽存在显著差异的品系间该基因的表达存在显著差异。基于对携带保守氨基酸终止突变和该基因中两个非同义突变且叶宽较窄的EMS突变体系的评估，这些数据表明该基因是玉米叶宽的关键调节因子。
