Kalia Vipin C, Patel Sanjay K S, Karthikeyan Kugalur K, Jeya Marimuthu, Kim In-Won, Lee Jung-Kul
Department of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Marine Biotechnology Division, National Institute of Ocean Technology, Chennai 600100, India.
Polymers (Basel). 2024 Feb 1;16(3):410. doi: 10.3390/polym16030410.
The total rate of plastic production is anticipated to surpass 1.1 billion tons per year by 2050. Plastic waste is non-biodegradable and accumulates in natural ecosystems. In 2020, the total amount of plastic waste was estimated to be 367 million metric tons, leading to unmanageable waste disposal and environmental pollution issues. Plastics are produced from petroleum and natural gases. Given the limited fossil fuel reserves and the need to circumvent pollution problems, the focus has shifted to biodegradable biopolymers, such as polyhydroxyalkanoates (PHAs), polylactic acid, and polycaprolactone. PHAs are gaining importance because diverse bacteria can produce them as intracellular inclusion bodies using biowastes as feed. A critical component in PHA production is the downstream processing procedures of recovery and purification. In this review, different bioengineering approaches targeted at modifying the cell morphology and synchronizing cell lysis with the biosynthetic cycle are presented for product separation and extraction. Complementing genetic engineering strategies with conventional downstream processes, these approaches are expected to produce PHA sustainably.
预计到2050年，塑料的年总产量将超过11亿吨。塑料垃圾不可生物降解，会在自然生态系统中累积。2020年，塑料垃圾总量估计为3.67亿吨，导致了难以管理的废物处理和环境污染问题。塑料由石油和天然气制成。鉴于化石燃料储备有限以及需要规避污染问题，重点已转向可生物降解的生物聚合物，如聚羟基脂肪酸酯（PHA）、聚乳酸和聚己内酯。PHA正变得越来越重要，因为多种细菌可以利用生物废料作为原料，将它们作为细胞内包涵体来生产。PHA生产中的一个关键组成部分是回收和纯化的下游加工过程。在这篇综述中，介绍了针对改变细胞形态以及使细胞裂解与生物合成周期同步的不同生物工程方法，用于产品的分离和提取。将基因工程策略与传统下游工艺相结合，预计这些方法能够可持续地生产PHA。
