Phytochemical Screening Using LC-MS to Study Antioxidant and Toxicity Potential of Methanolic Extracts of Bunge.

, a native medicinal plant of Central Asia, has a long history of traditional medicinal use; however, scientific research on its phytochemical and biological properties remains scarce. This paper aims to elucidate its chemical profile and assess its pharmacological potential through a comprehensive investigation of the phytochemical composition of stems and leaves using Liquid Chromatography-Mass Spectrometry (LC-MS), in conjunction with the assessment of its antioxidant (DPPH and ABTS) and cytotoxicity test on . Predominantly, glycosylated flavonoids were detected in stems and leaves extracts, notably including 8-Acetoxy-3',4',5,5'-tetrahydroxy-7-methoxy-3-α-L-rhamno-pyranosyloxyflavone, pyrifolin, and dehydroxypyrifolin. While the latter compound is exclusive to , the former compounds serve as shared chemical markers with other species. The methanolic extracts of leaves exhibited significant antioxidant capacity without toxicity against . This study contributes to current research through providing valuable insights into the chemical diversity and potential medicinal properties of this plant species.