Institute of Gene Biology Russian Academy of Sciences, 119334 Moscow, Russia.
Faculty of Biology, MSU-BIT University, Shenzhen 518172, China.
Viruses. 2024 Sep 3;16(9):1410. doi: 10.3390/v16091410.
Highly diverse phages infecting thermophilic bacteria of the genus have been isolated over the years from hot springs around the world. Many of these phages are unique, rely on highly unusual developmental strategies, and encode novel enzymes. The variety of phages is clearly undersampled, as evidenced, for example, by a paucity of phage-matching spacers in CRISPR arrays. Using water samples collected from hot springs in the Kunashir Island from the Kuril archipelago and from the Tsaishi and Nokalakevi districts in the Republic of Georgia, we isolated several distinct phages infecting laboratory strains of . Genomic sequence analysis of 11 phages revealed both close relatives of previously described phages isolated from geographically distant sites, as well as phages with very limited similarity to earlier isolates. Comparative analysis allowed us to predict several accessory phage genes whose products may be involved in host defense/interviral warfare, including a putative Type V CRISPR- system.
多年来，人们从世界各地的温泉中分离到了感染嗜热菌属的高度多样化噬菌体。这些噬菌体中有许多是独特的，依赖于非常不寻常的发育策略，并编码新的酶。噬菌体的多样性显然是未被充分采样的，例如，CRISPR 阵列中噬菌体匹配间隔物的缺乏就是证据。我们使用从千岛群岛的 Kunashir 岛和格鲁吉亚共和国的 Tsaishi 和 Nokalakevi 地区的温泉采集的水样，分离到了几种感染实验室菌株的噬菌体。对 11 种噬菌体的基因组序列分析揭示了与以前从地理上遥远的地点分离到的噬菌体密切相关的噬菌体，以及与早期分离物相似度非常有限的噬菌体。比较分析使我们能够预测几种辅助噬菌体基因，其产物可能参与宿主防御/抗病毒战争，包括一种假定的 Type V CRISPR 系统。
