Koupparis M A, Diamandis E P, Malmstadt H V
Clin Chim Acta. 1985 Jul 15;149(2-3):225-35. doi: 10.1016/0009-8981(85)90336-5.
We describe an adaptation of a microcomputer-controlled stopped-flow analyzer for automated analysis of albumin in serum. The immediate reaction between bromcresol green or purple and albumin is used to develop a routine procedure with 12 s reaction time. The approach shows excellent linearity to 68 g/l, good sensitivity, a relative SD of less than 0.5%, mean recovery 99.1%, high sample throughput (180 prediluted samples/h) and no significant interferences. The 'reaction time' related overestimation of albumin that appears in prolonged procedures is eliminated, and results with this method correlate well with those obtained using the more specific immunonephelometric method.
我们描述了一种对微机控制的停流分析仪的改进方法，用于血清中白蛋白的自动分析。利用溴甲酚绿或溴甲酚紫与白蛋白之间的即时反应，开发了一种反应时间为12秒的常规检测方法。该方法在白蛋白浓度达68 g/l时表现出良好的线性、高灵敏度、相对标准差小于0.5%、平均回收率99.1%、高样本通量（每小时180个预稀释样本）且无明显干扰。消除了在较长检测过程中出现的与“反应时间”相关的白蛋白高估现象，该方法所得结果与使用更特异的免疫比浊法获得的结果相关性良好。
