Br Med J (Clin Res Ed). 1985 Dec 7;291(6509):1601-5. doi: 10.1136/bmj.291.6509.1601.
Four hundred adults presenting with acute watery diarrhoea were entered into a randomised, placebo controlled, double blind clinical trial of berberine, tetracycline, and tetracycline and berberine to study the antisecretory and vibriostatic effects of berberine. Of 185 patients with cholera, those given tetracycline or tetracycline and berberine had considerably reduced volume and frequency of diarrhoeal stools, duration of diarrhoea, and volumes of required intravenous and oral rehydration fluid. Berberine did not produce an antisecretory effect. Analysis by factorial design equations, however, showed a reduction in diarrhoeal stools by one litre and a reduction in cyclic adenosine monophosphate concentrations in stools by 77% in the groups given berberine. Considerably fewer patients given tetracycline or tetracycline and berberine excreted vibrios in stools after 24 hours than those given berberine alone. Neither tetracycline nor berberine had any benefit over placebo in 215 patients with non-cholera diarrhoea.
400名出现急性水样腹泻的成年人参与了一项关于黄连素、四环素以及四环素与黄连素联用的随机、安慰剂对照、双盲临床试验，以研究黄连素的抗分泌和抑制弧菌作用。在185名霍乱患者中，给予四环素或四环素与黄连素联用的患者，其腹泻粪便的量和频率、腹泻持续时间以及所需静脉和口服补液量均显著减少。黄连素未产生抗分泌作用。然而，通过析因设计方程分析显示，给予黄连素的组腹泻粪便量减少1升，粪便中环磷酸腺苷浓度降低77%。给予四环素或四环素与黄连素联用的患者，24小时后粪便中排出弧菌的人数比仅给予黄连素的患者少得多。在215名非霍乱性腹泻患者中，四环素和黄连素与安慰剂相比均无任何益处。
