Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
J Mol Histol. 2024 Dec;55(6):1225-1238. doi: 10.1007/s10735-024-10263-9. Epub 2024 Oct 1.
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths and the fifth most common cancer worldwide. Brown algae appeared to be a rich source of efficient and safe agents against many life-threatening diseases like cancer. Thus, the scope of this study was to investigate the therapeutic effects of Turbinaria ornata against experimentally induced HCC in a rat model. Accordingly, forty male albino rats were divided into four groups. HCC was induced by intraperitoneal injection with diethylnitrosamine (DENA) followed by carbon tetrachloride (CCL). After four weeks of DENA + CCL injection and two weeks of treatment with Turbinaria ornata, rats were sacrificed to collect hepatic tissue and blood samples for histopathological observations and various biochemical markers such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alpha-fetoprotein (AFP), urea, creatinine, albumin (ALB), and alkaline phosphatase (ALP). Rats that were injected for four weeks with DENA + CCL showed a significant increase in AFP levels, transforming growth factor-beta (TGF-β) and tumor necrosis factor-alpha (TNF-α), as well as a high percentage of malignant changes in hepatic tissues. The extension of malignant changes in the rat liver tissues was markedly reduced using Turbinaria ornata, as the treatment displayed liver patterns similar to that of the normal control rats. Furthermore, rats with HCC fed with Turbinaria ornata extract for two weeks showed decreasing levels of TGF-β and TNF-α. These findings demonstrate that Turbinaria ornata supplement can prevent HCC development in hepatic rats; however, the exact mechanism requires further investigation.
肝细胞癌 (HCC) 是癌症相关死亡的第三大原因，也是全球第五大最常见的癌症。褐藻似乎是一种丰富的有效且安全的药物来源，可以对抗许多危及生命的疾病，如癌症。因此，本研究的范围是研究 Turbinaria ornata 对实验诱导的 HCC 大鼠模型的治疗作用。
相应地，将 40 只雄性白化大鼠分为四组。通过腹腔注射二乙基亚硝胺 (DENA) 继以四氯化碳 (CCL) 诱导 HCC。在 DENA+CCL 注射四周和 Turbinaria ornata 治疗两周后，处死大鼠以收集肝组织和血液样本，进行组织病理学观察和各种生化标志物，如天冬氨酸转氨酶 (AST)、丙氨酸转氨酶 (ALT)、甲胎蛋白 (AFP)、尿素、肌酐、白蛋白 (ALB) 和碱性磷酸酶 (ALP)。
用 DENA+CCL 注射四周的大鼠 AFP 水平、转化生长因子-β (TGF-β) 和肿瘤坏死因子-α (TNF-α) 显著升高，肝组织恶性变化比例高。用 Turbinaria ornata 治疗显著减少了大鼠肝组织恶性变化的扩展，因为治疗显示出与正常对照大鼠相似的肝脏模式。此外，用 Turbinaria ornata 提取物喂养两周 HCC 大鼠的 TGF-β 和 TNF-α 水平降低。
这些发现表明 Turbinaria ornata 补充剂可以预防大鼠肝 HCC 的发生；然而，确切的机制需要进一步研究。
