拳参根提取物抑制二乙基亚硝胺处理大鼠的肝癌。
Rheum palmatum root extract inhibits hepatocellular carcinoma in rats treated with diethylnitrosamine.
机构信息
Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Menoufia Province, Egypt.
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany.
出版信息
J Pharm Pharmacol. 2018 Jun;70(6):821-829. doi: 10.1111/jphp.12899. Epub 2018 Mar 9.
OBJECTIVES
The aim of this study was to investigate the potential anticancer properties of a methanol extract of Rheum palmatum roots against diethylnitrosamine (DENA)-induced hepatocellular carcinoma (HCC) in rats and to characterize its phytoconstituents.
METHODS
HPLC-PDA-MS/MS was used to profile the secondary metabolites in R. palmatum root extract. HCC was induced using diethylnitrosamine (DENA). The activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT), alpha-fetoprotein (AFP), total proteins, serum albumin and serum globulin was determined. DNA fragmentation and histopathological examination and GST-P immunostaining were also studied.
KEY FINDINGS
LC-MS/MS analysis identified 16 compounds belonging to anthraquinones, flavonoids and tannins. The root extract significantly reduced the elevated liver enzymes ALT and AST and increased total proteins, albumin and globulin in HCC-rats. Also, the tumour markers AFP and GGT levels were significantly reduced in HCC-rats treated with the extract. In addition, the extract significantly reduced elevated DNA fragmentation and decreased the numbers and areas of GST-P positive putative foci in HCC-rats.
CONCLUSIONS
Rheum palmatum is a potential candidate to be explored for the treatment of hepatocellular carcinoma.
目的
本研究旨在探讨大黄根甲醇提取物对二乙基亚硝胺（DENA）诱导的大鼠肝癌（HCC）的潜在抗癌特性，并对其植物成分进行表征。
方法
采用高效液相色谱-光电二极管阵列-串联质谱联用技术（HPLC-PDA-MS/MS）对大黄根提取物中的次生代谢产物进行分析。采用二乙基亚硝胺（DENA）诱导肝癌。测定血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）和γ-谷氨酰转移酶（GGT）、甲胎蛋白（AFP）、总蛋白、血清白蛋白和血清球蛋白的活性。还研究了 DNA 片段化、组织病理学检查和 GST-P 免疫染色。
主要发现
LC-MS/MS 分析鉴定出 16 种化合物，属于蒽醌类、类黄酮类和鞣质类。根提取物可显著降低 HCC 大鼠升高的肝酶 ALT 和 AST，并增加 HCC 大鼠的总蛋白、白蛋白和球蛋白。此外，提取物还可显著降低 HCC 大鼠中升高的 AFP 和 GGT 水平。此外，该提取物可显著降低升高的 DNA 片段化水平，并减少 GST-P 阳性假定病灶的数量和面积。
结论
大黄是治疗肝癌的潜在候选药物。
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