Biological Science Research, Kao Corporation, Tochigi, Japan.
Human Health Care Product Research, Kao Corporation, Tokyo, Japan.
PLoS One. 2024 Oct 1;19(10):e0311372. doi: 10.1371/journal.pone.0311372. eCollection 2024.
Adult zebrafish are commonly used as disease models in biomedical research, but unlike in other model organisms such as rodents, there is no simple method for distinguishing individuals, even though the importance of individual differences is recognized in such research. We developed a side viewing device that can be used to capture images of stripe patterns and identified eight distinct components of stripe patterns on the caudal and anal fins that allowed us to distinguish individual fish. We found that the stripe patterns were consistent for at least 8 weeks in males and females of two lines of wild-type zebrafish. These results suggest that individual adult zebrafish can be distinguished in an easy and non-invasive manner, allowing researchers to incorporate individual differences in biomedical research as in rodent models.
成年斑马鱼通常被用作生物医学研究中的疾病模型，但与其他模式生物（如啮齿动物）不同，即使在这种研究中已经认识到个体差异的重要性，但仍然没有一种简单的方法可以区分个体。我们开发了一种侧视装置，可以用来捕捉条纹图案的图像，并确定了尾鳍和臀鳍上的 8 个独特的条纹图案组成部分，这些部分可以帮助我们区分个体鱼类。我们发现，在两条野生型斑马鱼的雄性和雌性中，条纹图案至少在 8 周内保持一致。这些结果表明，可以以简单且非侵入性的方式来区分成年斑马鱼个体，从而使研究人员能够像在啮齿动物模型中那样将个体差异纳入生物医学研究中。
