Abdullah Fuad O
Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq.
Department of Pharmacognosy, Faculty of Pharmacy, Tishk International University, Erbil, Iraq.
Nat Prod Res. 2024 Oct 1:1-6. doi: 10.1080/14786419.2024.2407504.
Natural extract plays a crucial role in our lives, major compound in the n-hexane (AR-H) and the ethyl acetate (AR-E) extracts was n-hexadecanoic acid, and of the methanol extract (AR-M) was pentacosane. The AR-M extract had a strong ability to induce mRNA expression of an inflammatory cytokine, IL-6, as an M1-like macrophage subset compared to the negative control (DMSO-treated cells). In contrast, AR-E treatment showed strong anti-inflammatory activity against macrophages. The AR-H extract had a moderate inflammatory effect against macrophages. The IC50 results of the anticancer assays ranged from 58.29 to 451.03 μg/mL for the three extracts. The anticancer action of the AR-E extract against U-87MG cells was higher (58.29 μg/mL) than that of AR-H and AR-M (156.38 and 196.14 μg/mL, respectively). The greater cytotoxicity effect observed with the AR-E extract against U-87MG can be linked to its high content of hexadecanoic acid (32.49%) and linolenic acid (12.90%).
天然提取物在我们的生活中起着至关重要的作用，正己烷提取物（AR-H）和乙酸乙酯提取物（AR-E）中的主要化合物是十六烷酸，而甲醇提取物（AR-M）中的主要化合物是二十五烷。与阴性对照（二甲基亚砜处理的细胞）相比，AR-M提取物作为M1样巨噬细胞亚群具有很强的诱导炎性细胞因子IL-6 mRNA表达的能力。相比之下，AR-E处理对巨噬细胞显示出强大的抗炎活性。AR-H提取物对巨噬细胞具有中等程度的炎症作用。三种提取物的抗癌试验IC50结果在58.29至451.03μg/mL之间。AR-E提取物对U-87MG细胞的抗癌作用（58.29μg/mL）高于AR-H和AR-M（分别为156.38和196.14μg/mL）。观察到AR-E提取物对U-87MG具有更大的细胞毒性作用，这可能与其十六烷酸（32.49%）和亚麻酸（12.90%）的高含量有关。
