新鲜人血浆对硫胺素焦磷酸的去磷酸化作用。
Dephosphorylation of thiamin pyrophosphate by fresh human plasma.
作者信息
Thom J Y, Davis R E, Icke G C
出版信息
Int J Vitam Nutr Res. 1985;55(3):269-73.
PMID:3935590
Abstract
Thiamin pyrophosphate was added to human plasma and serum. After various incubations, aliquots were assayed by the thiochrome method. This revealed that fresh human plasma dephosphorylated thiamin pyrophosphate by a reaction that was time and temperature dependent and inhibited by the presence of ethylenediaminetetraacetic acid (EDTA). Fresh plasma did not dephosphorylate pyridoxal-5-phosphate nor the protein bound thiamin pyrophosphate of red cells.
摘要
将硫胺素焦磷酸添加到人体血浆和血清中。经过不同时间的孵育后，取等分试样采用硫色素法进行测定。结果显示，新鲜人体血浆通过一种与时间和温度相关且受乙二胺四乙酸（EDTA）抑制的反应使硫胺素焦磷酸去磷酸化。新鲜血浆不会使磷酸吡哆醛或红细胞中与蛋白质结合的硫胺素焦磷酸去磷酸化。
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