Konttinen Y, Bergroth V, Nykänen P
Scand J Immunol. 1985 Nov;22(5):503-7. doi: 10.1111/j.1365-3083.1985.tb01909.x.
Monoclonal antibodies were used in avidin-biotin-peroxidase complex staining for activation marker analysis of rheumatoid synovial fluid cells. Although Ia expression indicates T cell activation, cells displaying receptors for interleukin 2 (Tac)-and transferrin receptor (T9)- positive proliferating cells were relatively few. Similarly, activated terminal effector cells of suppressor/cytotoxic nature were scarce in rheumatoid synovial fluid, as suggested by a low expression of Tac and 4F2 markers. The in vivo situation in the rheumatoid arthritic (RA) joint does not seem to be due to the inability of synovial fluid lymphocytes to become activated, because mitogen stimulation in vitro, in spite of a low proliferative response, induced expression of all the activation markers studied. The relevance of the present observations to the down-regulation of the active, inflammatory-immune response in situ is speculative, but the data show that in spite of T-cell activation and Ia expression, activated terminal effector cells of suppressor/cytotoxic nature are few in the RA joint in vivo.
单克隆抗体用于抗生物素蛋白-生物素-过氧化物酶复合物染色，以分析类风湿性滑液细胞的活化标志物。尽管Ia表达表明T细胞活化，但显示白细胞介素2受体（Tac）和转铁蛋白受体（T9）的阳性增殖细胞相对较少。同样，类风湿性滑液中具有抑制/细胞毒性性质的活化终末效应细胞也很少，Tac和4F2标志物的低表达表明了这一点。类风湿性关节炎（RA）关节的体内情况似乎并非由于滑液淋巴细胞无法被激活，因为尽管体外有丝分裂原刺激的增殖反应较低，但仍能诱导所研究的所有活化标志物的表达。目前观察结果与原位活性炎症免疫反应下调的相关性尚属推测，但数据表明，尽管存在T细胞活化和Ia表达，但在体内RA关节中具有抑制/细胞毒性性质的活化终末效应细胞很少。
