Ofosu-Appiah W A, Warrington R J, Wilkins J A
Rheumatic Disease Unit Research Laboratory, University of Manitoba, Winnipeg, Canada.
Rheumatol Int. 1987;7(4):147-51. doi: 10.1007/BF00270362.
Lymphocytes from peripheral blood (PBL) and synovial fluid (SFL) were obtained from patients with rheumatoid arthritis (RA) and cloned under limiting-dilution conditions without prior activation but in the presence of exogenous interleukin (IL)-2. The precursor frequencies of such in vivo activated IL-2-responsive cells were higher in RA SFL (1/83) than in RA PBL (1/201) or normal PBL (1/377). These HLA-Dr/Ia-positive clones expressed T-cell markers CD3 and T101 and were either CD4- or CD8-positive but lacked NK markers CD11, CD16, and HNK-1. All such clones were cytotoxic for NK-sensitive K562 targets and NK-insensitive Raji cell targets. These cells, which most closely resemble nonmajor histocompatibility complex (MHC) restricted cytotoxic T (CTL) cells, are present with increased frequency in RA synovial fluids.
从类风湿性关节炎（RA）患者中获取外周血淋巴细胞（PBL）和滑液淋巴细胞（SFL），并在有限稀释条件下进行克隆，无需预先激活，但需添加外源性白细胞介素（IL）-2。此类体内激活的IL-2反应性细胞的前体频率在RA SFL中（1/83）高于RA PBL（1/201）或正常PBL（1/377）。这些HLA-Dr/Ia阳性克隆表达T细胞标志物CD3和T101，要么是CD4阳性，要么是CD8阳性，但缺乏NK标志物CD11、CD16和HNK-1。所有此类克隆对NK敏感的K562靶细胞和NK不敏感的Raji细胞靶细胞均具有细胞毒性。这些与非主要组织相容性复合体（MHC）限制性细胞毒性T（CTL）细胞最为相似的细胞，在RA滑液中的频率增加。
