Kim Jung Heon, Kim Jae Sik, Choi Noorie, Koh Jiwon, Jeon Yoon Kyung, Chang Ji Hyun, Hwang Eung Soo, Kim Il Han
Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea.
Department of Radiation Oncology, Soonchunhyang University Seoul Hospital, Soonchunhyang University College of Medicine, Seoul, Korea.
Cancer Res Treat. 2025 Apr;57(2):580-589. doi: 10.4143/crt.2024.689. Epub 2024 Sep 30.
It is well known that the majority of the extranodal marginal zone lymphomas of mucosa-associated lymphoid tissues (MALT lymphomas) are associated with microbiota, e.g., gastric MALT lymphoma with Helicobacter pylori. In general, they are very sensitive to low-dose radiotherapy and chemotherapeutic agents. The microbiota profile is not clearly elucidated in bronchus-associated lymphoid tissue (BALT) lymphoma, a rare type of MALT lymphoma in the lung. Thus, this study aimed to clarify the intratumor microbiome in BALT lymphoma using the third-generation next-generation sequencing (NGS) method.
DNAs were extracted from 12 formalin-fixed paraffin-embedded (FFPE) tumor tissues obtained from BALT lymphoma patients diagnosed between 1990 and 2016. 16S rRNA gene was amplified by polymerase chain reaction. Amplicons were sequenced using a Nanopore platform. Next-generation sequencing analysis was performed to assess microbial profiles. For comparison, FFPE specimens from nine non-cancerous lung tissues were also analyzed.
Specific bacterial families including Burkholderiaceae, Bacillaceae, and Microbacteriaceae were associated with BALT lymphoma by a linear discriminant analysis effect size approach. Although the number of specimens was limited, BALT lymphomas exhibited significantly higher microbial abundance and diversity with distinct microbial composition patterns and correlation networks than non-cancerous lung tissues.
This study provides the first insight into intratumor microbiome in BALT lymphoma using the third-generation NGS method. A distinct microbial composition suggests the presence of a unique tumor microenvironment of BALT lymphoma.
众所周知，大多数黏膜相关淋巴组织的结外边缘区淋巴瘤（MALT淋巴瘤）与微生物群有关，例如胃MALT淋巴瘤与幽门螺杆菌有关。一般来说，它们对低剂量放疗和化疗药物非常敏感。在支气管相关淋巴组织（BALT）淋巴瘤（一种罕见的肺部MALT淋巴瘤）中，微生物群谱尚未明确阐明。因此，本研究旨在使用第三代新一代测序（NGS）方法阐明BALT淋巴瘤中的肿瘤内微生物组。
从1990年至2016年诊断的BALT淋巴瘤患者的12份福尔马林固定石蜡包埋（FFPE）肿瘤组织中提取DNA。通过聚合酶链反应扩增16S rRNA基因。使用纳米孔平台对扩增子进行测序。进行新一代测序分析以评估微生物谱。为了进行比较，还分析了来自9份非癌性肺组织的FFPE标本。
通过线性判别分析效应大小方法，包括伯克霍尔德菌科、芽孢杆菌科和微杆菌科在内的特定细菌家族与BALT淋巴瘤相关。尽管样本数量有限，但与非癌性肺组织相比，BALT淋巴瘤表现出显著更高的微生物丰度和多样性，具有独特的微生物组成模式和相关网络。
本研究首次使用第三代NGS方法对BALT淋巴瘤中的肿瘤内微生物组进行了深入研究。独特的微生物组成表明BALT淋巴瘤存在独特的肿瘤微环境。
