Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Para, Brazil.
Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
PLoS One. 2024 Oct 4;19(10):e0309390. doi: 10.1371/journal.pone.0309390. eCollection 2024.
This study aimed to investigate the impact of minocycline on the alveolar bone in experimental periodontitis in rats. Thirty Wistar rats were randomly assigned to three groups: control without periodontitis; experimental periodontitis induced by ligature; experimental periodontitis + intraperitoneal administration minocycline for seven days. Ligatures remained in place in both periodontitis groups for 14 days. At the end of the experiment, the animals were euthanized and one hemimandible underwent micro-computed tomography (micro-CT) analysis to assess vertical bone loss and alveolar bone quality. Histopathological analysis was performed on the other hemimandible. Statistical analysis was performed using ANOVA with Tukey's post-test (p<0.05). The results showed a significant reduction in vertical bone loss in the animals treated with minocycline compared with untreated animals. Minocycline also preserved the alveolar bone thickness, number, spacing, and bone volume to tissue volume ratio. Histopathological analysis indicated that minocycline reduced bone resorption, decreased inflammatory response, and maintained the bone collagen fibers. This study demonstrated the effectiveness of minocycline in reducing vertical bone loss and preserved bone quality in rats with experimental periodontitis. The results of this study indicate that minocycline has the potential to serve as an additional treatment option for periodontitis. However, further research is warranted to assess the efficacy and safety of minocycline use in patients with periodontitis.
本研究旨在探讨米诺环素对实验性牙周炎大鼠牙槽骨的影响。将 30 只 Wistar 大鼠随机分为三组：无牙周炎对照组；结扎诱导的牙周炎实验组；结扎诱导的牙周炎+腹腔内给予米诺环素治疗组，共治疗 7 天。两组牙周炎大鼠的结扎均保留 14 天。实验结束时，处死动物，一侧下颌骨进行 micro-CT 分析，评估垂直骨丧失和牙槽骨质量；另一侧下颌骨进行组织学分析。采用 ANOVA 与 Tukey 事后检验进行统计学分析（p<0.05）。结果显示，与未治疗动物相比，米诺环素治疗的动物垂直骨丧失显著减少。米诺环素还能保持牙槽骨厚度、数量、间距和骨体积/组织体积比。组织学分析表明，米诺环素减少了骨吸收，降低了炎症反应，维持了骨胶原纤维。本研究表明米诺环素在减少实验性牙周炎大鼠垂直骨丧失和维持骨质量方面具有有效性。然而，需要进一步的研究来评估米诺环素在牙周炎患者中的疗效和安全性。
