Tarigan Desi Natalia, Tarigan Yenni Gustiani, Prakasita Vinsa Cantya, Prasetyaningsih Aniek, Kachingwe Baxter H K
Faculty of Biotechnology, Duta Wacana Christian University, Yogyakarta, Indonesia.
Faculty of Pharmacy and Health Sciences, Sari Mutiara Indonesia University, Medan, Indonesia.
J Exp Pharmacol. 2024 Oct 4;16:339-350. doi: 10.2147/JEP.S479862. eCollection 2024.
Rope bamboo () is traditionally used for medicinal purposes, and extracts from stem leaves and shoots have been shown to possess antioxidant and anti-inflammatory activity. Thus, this study looked at the potential compounds present in and the usefulness of Rope bamboo liquid smoke preparations in the wound healing process in mice.
The fingerprinting of the liquid smoke was done by liquid chromatography-mass spectrometry. In-vivo experiments were conducted to observe the diameter and percentage of wound healing in mice for 14 days using topical formulations containing liquid smoke concentrations of 100%, 50%, 25%, positive control and negative control. Statistical analyses were conducted using the Kruskal-Wallis test and Spearman correlation.
The phytochemical fingerprint showed the presence of alkaloids, flavonoids, vitamins, phenols, and lipids. The 100% undiluted liquid smoke accelerated wound healing faster compared to 50% and 25% dilutions. The differences in wound diameters were statistically significant across treatments having a of 0.020 and dose-dependent ().
Liquid smoke acceleration of the wound healing process was dose-dependent compared to controls. This dose-dependency indicates that the wound healing effects were probably due to the anti-inflammatory, antioxidant, and antimicrobial activities of the elucidated constituents of Rope bamboo liquid smoke.
藤竹传统上用于药用，其茎叶和嫩枝提取物已显示具有抗氧化和抗炎活性。因此，本研究探讨了藤竹烟熏液制剂中存在的潜在化合物及其在小鼠伤口愈合过程中的作用。
通过液相色谱 - 质谱联用对烟熏液进行指纹图谱分析。进行体内实验，使用含100%、50%、25%烟熏液浓度的局部制剂、阳性对照和阴性对照，观察小鼠伤口愈合的直径和百分比，持续14天。使用Kruskal - Wallis检验和Spearman相关性进行统计分析。
植物化学指纹图谱显示存在生物碱、黄酮类化合物、维生素、酚类和脂质。与50%和25%稀释液相比，100%未稀释的烟熏液能更快地加速伤口愈合。各处理组伤口直径差异具有统计学意义（P = 0.020）且呈剂量依赖性（r = 0.876）。
与对照组相比，烟熏液对伤口愈合过程的加速作用呈剂量依赖性。这种剂量依赖性表明伤口愈合效果可能归因于藤竹烟熏液中已阐明成分的抗炎、抗氧化和抗菌活性。
