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基于 16s rRNA 测序的诺如病毒感染患儿肠道菌群失调研究。

Investigation of gut microbiota disorders in norovirus infected children patients based on 16s rRNA sequencing.

机构信息

Comprehensive Technical Service Center, Taizhou Customs, Taizhou City, Zhejiang, China.

Department of Integrated Traditional Chinese and Western Medicine, Taizhou Municipal Hospital, Taizhou City, Zhejiang, China.

出版信息

Ann Med. 2024 Dec;56(1):2412834. doi: 10.1080/07853890.2024.2412834. Epub 2024 Oct 10.

DOI:10.1080/07853890.2024.2412834
PMID:39387550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11469441/
Abstract

BACKGROUND

Norovirus is the leading cause of sporadic viral gastroenteritis cases and outbreaks. Gut microbiota plays a key role in maintaining immune homeostasis. We aimed to investigate the composition and functional effects of gut microbiota in children infected with norovirus.

METHODS

Stool samples were collected from 31 children infected with norovirus and 25 healthy children. The gut microbiota was analyzed by 16S rRNA gene sequencing, followed by composition, correlation network, functional and phenotype prediction analyses.

RESULTS

Gut microbiota in children infected with norovirus was characterized by lower species richness and diversity. is the dominant gut microbiota specie in norovirus infection. was significantly lower in norovirus infection. There was a positive correlation between , , , , , , and . Functionally, secondary metabolites biosynthesis, transport and catabolism, selenocysteine lyase and peroxiredoxin were the most significantly higher functional compositions of gut microbiota in norovirus infection. However, sn-glycerol-1-phosphate dehydrogenase and fermentation were the most significantly lower functional compositions in norovirus infection group. Phenotype analysis showed that Contains_Mobile_Elements had the highest level of phenotypes in the gut microbiota of norovirus infection.

CONCLUSION

Norovirus infection may lead to dysregulation of the gut microbiome in children.

摘要

背景

诺如病毒是散发性病毒性胃肠炎病例和暴发的主要原因。肠道微生物群在维持免疫稳态方面发挥着关键作用。我们旨在研究感染诺如病毒的儿童肠道微生物群的组成和功能影响。

方法

收集了 31 名感染诺如病毒的儿童和 25 名健康儿童的粪便样本。通过 16S rRNA 基因测序分析肠道微生物群，然后进行组成、相关网络、功能和表型预测分析。

结果

感染诺如病毒的儿童肠道微生物群的物种丰富度和多样性较低。是感染诺如病毒时的主要肠道微生物群种类。在诺如病毒感染中显著降低。、、、、、、和之间存在正相关关系。在功能上，次生代谢物的生物合成、运输和分解、硒代半胱氨酸裂解酶和过氧化物酶是感染诺如病毒时肠道微生物群中最显著升高的功能成分。然而，sn-甘油-1-磷酸脱氢酶和发酵是感染诺如病毒组中最显著降低的功能成分。表型分析表明，感染诺如病毒时肠道微生物群中含有移动元件的表型水平最高。

结论

诺如病毒感染可能导致儿童肠道微生物群失调。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/d1dcfa39c9ae/IANN_A_2412834_F0009_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/ba33d60db959/IANN_A_2412834_F0001_C.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/7f54c1c5a51b/IANN_A_2412834_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/4f4c7d84b63d/IANN_A_2412834_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/65043fac1ceb/IANN_A_2412834_F0008_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/d1dcfa39c9ae/IANN_A_2412834_F0009_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/ba33d60db959/IANN_A_2412834_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/ed0cc991a369/IANN_A_2412834_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/4ac8c8d932be/IANN_A_2412834_F0003_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/8ef4e201020e/IANN_A_2412834_F0004_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/209bc172fb60/IANN_A_2412834_F0005_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/7f54c1c5a51b/IANN_A_2412834_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/4f4c7d84b63d/IANN_A_2412834_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/65043fac1ceb/IANN_A_2412834_F0008_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b04/11469441/d1dcfa39c9ae/IANN_A_2412834_F0009_C.jpg

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