Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology, Qingdao, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Microbiol Spectr. 2022 Aug 31;10(4):e0016122. doi: 10.1128/spectrum.00161-22. Epub 2022 Jul 5.
Human norovirus (HuNoV) is a major foodborne pathogen that causes acute viral gastroenteritis, and oysters are one of the main carriers of HuNoV transmission. While progress has been made toward understanding the pattern of oyster-bioaccumulated HuNoV, the response of oysters to HuNoV bioaccumulation, including changes in gene expression and gut microbiota, is unclear. In this study, histo-blood group antigen (HBGA)-like molecule expression and gene regulation features and the HuNoV-microbiome interactions of oysters during HuNoV bioaccumulation were characterized. With the prolongation of bioaccumulation time, the HuNoV content and expression of type A HBGA-like molecules in oysters increased and stabilized. HuNoV also altered the expression of immunity- and glycosphingolipid biosynthesis-related genes. Prolonged bioaccumulation of HuNoV can reduce the abundance and change the composition of the oyster gut microbiota. In particular, with the extension of bioaccumulation time, the abundance of , , , , , , and decreased, while the abundance of and increased. This study provides potential candidates for identifying functional genes involved in the bioaccumulation of HuNoV in oysters. More importantly, it provides the first description of the changes in gut microbiota during HuNoV bioaccumulation in oysters. The role of the oyster gut microbiota in HuNoV bioaccumulation is poorly understood. This study revealed, for the first time, the changes in gut microbiota and gene expression of oysters with HuNoV bioaccumulation. This study enriches the understanding of the impact of HuNoV bioaccumulation on oysters and provides a new direction for the study of the molecular mechanism of HuNoV bioaccumulation in oysters.
人诺如病毒（HuNoV）是一种主要的食源性病原体，可引起急性病毒性肠胃炎，而牡蛎是 HuNoV 传播的主要载体之一。尽管人们在了解牡蛎生物累积 HuNoV 的模式方面取得了进展，但牡蛎对 HuNoV 生物累积的反应，包括基因表达和肠道微生物群的变化，尚不清楚。在这项研究中，我们对牡蛎在 HuNoV 生物累积过程中组织血型抗原（HBGA）样分子表达和基因调控特征以及 HuNoV-微生物组相互作用进行了研究。随着生物累积时间的延长，牡蛎中 HuNoV 含量和 A 型 HBGA 样分子的表达增加并趋于稳定。HuNoV 还改变了与免疫和糖脂生物合成相关的基因表达。HuNoV 的长期生物累积会降低牡蛎肠道微生物群的丰度并改变其组成。特别是，随着生物累积时间的延长， 、 、 、 、 、 和 的丰度降低，而 和 的丰度增加。本研究为鉴定牡蛎中 HuNoV 生物累积相关功能基因提供了潜在候选基因。更重要的是，它首次描述了牡蛎中 HuNoV 生物累积过程中肠道微生物群的变化。牡蛎肠道微生物群在 HuNoV 生物累积中的作用尚不清楚。本研究首次揭示了 HuNoV 生物累积对牡蛎肠道微生物群和基因表达的影响。本研究丰富了对 HuNoV 生物累积对牡蛎影响的认识，并为研究牡蛎中 HuNoV 生物累积的分子机制提供了新的方向。
