Key Lab. of Oral Diseases Research of Anhui Province, College & Hospital of Stomatology, Anhui Medical University, 81 Meishan Road, Hefei 230032, China; The Institute of Oral Science, Department of Stomatology, Longgang Otorhinolaryngology Hospital of Shenzhen, Shenzhen 518172, China.
The Institute of Oral Science, Department of Stomatology, Longgang Otorhinolaryngology Hospital of Shenzhen, Shenzhen 518172, China.
Int J Biol Macromol. 2024 Nov;281(Pt 3):136454. doi: 10.1016/j.ijbiomac.2024.136454. Epub 2024 Oct 9.
Wide used guided bone regeneration (GBR) membrane materials, such as collagen, Teflon, and other synthesized polymers, present a great challenge in term of integrating the mechanical property and degradation rate when addressing critical bone defects. Therefore, inspired by the distinctive architecture of fish scales, this study utilized epigallocatechin gallate to modify decellularized fish scales following biomimetic mineralization to fabricate a GBR membrane that mimics the structure of lamellar bone. The structure, physical and chemical properties, and biological functions of the novel GBR membrane were evaluated. Results indicate that the decellularized fish scale with 5 remineralization cycles (5R-E-DCFS) exhibited a composite and structure similar to natural bone and had a special functionally gradient mineral contents character, demonstrating excellent mechanical properties, hydrophilicity, and degradation properties. In vitro, the 5R-E-DCFS membrane exhibited excellent cytocompatibility promoting Sprague-Dawley (SD) rat bone marrow mesenchymal stem cell proliferation and differentiation up-regulating the expression of osteogenic-related genes and proteins. Furthermore, in vivo, the 5R-E-DCFS membrane promoted the critical skull bone defects of SD rats repairment and regeneration. Therefore, this innovative biomimetic membrane holds substantial clinical potential as an ideal GBR membrane with mechanical properties for space-making and suitable degradation rate for bone regeneration to manage bone defects.
广泛应用的引导骨再生（GBR）膜材料，如胶原、特氟龙和其他合成聚合物，在解决临界骨缺损时，其机械性能和降解速率的整合方面存在很大挑战。因此，受鱼鳞独特结构的启发，本研究采用表没食子儿茶素没食子酸酯（EGCG）对脱细胞鱼鳞进行仿生矿化修饰，以制备模仿板层骨结构的 GBR 膜。对新型 GBR 膜的结构、物理化学性质和生物学功能进行了评估。结果表明，经过 5 次再矿化循环的脱细胞鱼鳞（5R-E-DCFS）具有类似于天然骨的复合结构和相似结构，具有特殊的功能梯度矿化含量特征，表现出优异的机械性能、亲水性和降解性能。体外实验中，5R-E-DCFS 膜表现出良好的细胞相容性，促进 Sprague-Dawley（SD）大鼠骨髓间充质干细胞的增殖和分化，上调成骨相关基因和蛋白的表达。此外，在体内，5R-E-DCFS 膜促进了 SD 大鼠临界颅骨缺损的修复和再生。因此，这种创新的仿生膜具有很大的临床潜力，可作为一种理想的 GBR 膜，具有空间形成的机械性能和适合的降解速率，可用于骨再生以治疗骨缺损。
