Alaneme Kenneth Kanayo, Fagbayi Sandra Boluwatife, Nwanna Esther Emem, Ojo Ochuko Mary
Materials Design and Structural Integrity Group, Department of Metallurgical and Materials Engineering, The Federal University of Technology Akure, P.M.B. 704, Ondo State, Nigeria.
Centre for Nanoengineering and Advanced Materials, School of Mining, Metallurgy and Chemical Engineering, Faculty of Engineering & the Built Environment, University of Johannesburg, South Africa.
Heliyon. 2024 Sep 24;10(19):e38378. doi: 10.1016/j.heliyon.2024.e38378. eCollection 2024 Oct 15.
The biosafety of thermally calcined poultry manure as a hydroxyapatite source for potential use as bone-making material was investigated in this study. In vitro assays were used to determine the sensitivity of the antioxidant properties to the thermal calcination temperature used to process the poultry manure (750, 800, and 850 ). The effect of the extract of both calcined poultry manure (local) and analytical grade hydroxyapatite (foreign) at various concentrations of 100%-25 % inclusion at (100 mg/kg) body weight intubation for 21 days on kidney, liver, and serum of animal model used was assessed. The results show that the thermally calcined poultry manure-derived hydroxyapatite generally possessed good antioxidant properties with the poultry manure treated at 750 having the most promising antioxidant properties compared to those treated at 800 and 850 , and hence a more likely improved anti-toxicity potential. The various blends of the analytical high-grade hydroxyapatite and thermally calcined poultry manure hydroxyapatite samples are safe compared to the normal control rats with regards hepatic function and renal function parameters with the equal blend of analytical high grade and thermally calcined poultry manure-derived hydroxyapatite (1:1) possessing the lowest activity concentrations. In addition, the enzymatic (glutathione peroxidase) and non-enzymatic (reduced glutathione) antioxidant concentrations of the experimental animals administered the varied compositions of the analytical high grade and thermally calcined poultry manure-derived hydroxyapatite, were lower when compared to normal control rats. The microbiological evaluation suggests that the calcined poultry manure inclusion at various concentrations could not pose a negative effect on various pathology in the liver, kidney, and blood.
本研究调查了热煅烧家禽粪便作为羟基磷灰石来源用作制骨材料的生物安全性。体外试验用于确定抗氧化性能对用于处理家禽粪便的热煅烧温度（750、800和850 ）的敏感性。评估了煅烧家禽粪便（本地）提取物和分析级羟基磷灰石（国外）提取物在不同浓度（100%-25%）、以100mg/kg体重插管21天对所用动物模型的肾脏、肝脏和血清的影响。结果表明，热煅烧家禽粪便衍生的羟基磷灰石一般具有良好的抗氧化性能，与800和850 处理的相比，750 处理的家禽粪便具有最有前景的抗氧化性能，因此更有可能提高抗毒性潜力。与正常对照大鼠相比，分析级高品位羟基磷灰石和热煅烧家禽粪便羟基磷灰石样品的各种混合物在肝功能和肾功能参数方面是安全的，分析级高品位和热煅烧家禽粪便衍生的羟基磷灰石的等量混合物（1:1）具有最低的活性浓度。此外，与正常对照大鼠相比，给予分析级高品位和热煅烧家禽粪便衍生的羟基磷灰石不同组成的实验动物的酶促（谷胱甘肽过氧化物酶）和非酶促（还原型谷胱甘肽）抗氧化剂浓度较低。微生物学评估表明，不同浓度的煅烧家禽粪便掺入物不会对肝脏、肾脏和血液中的各种病变产生负面影响。
