Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa, 240-8501, Japan.
Institute of Advanced Sciences, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa, 240-8501, Japan.
Sci Rep. 2024 Oct 13;14(1):23935. doi: 10.1038/s41598-024-74962-9.
Oxytocin has various effects ranging from promoting labor in pregnant women to alleviating stress. Recently, we reported the hair growth-promoting effects of oxytocin in hair follicle organoids. However, its clinical application faces challenges such as rapid degradation in vivo and poor permeability due to its large molecular weight. Therefore, in this study, we investigated the effects of the oxytocin receptor (OXTR) agonists WAY267464 and LIT001 as alternatives to oxytocin on hair growth. Human dermal papilla (DP) cells were cultured in WAY267464 or LIT001-supplemented medium. The addition of WAY267464 and LIT001 increased the expression of hair growth-related genes in DP cells. We tested the hair growth-promoting effects of WAY267464 and LIT001 using hair follicle organoids in vitro and found that they significantly promoted hair follicle sprouting. Thus, our findings indicate that WAY267464 and LIT001 are potential hair growth agents and may encourage further research on the development of novel hair growth agents targeting OXTR in patients with alopecia.
催产素具有多种作用，从促进孕妇分娩到缓解压力。最近，我们报道了催产素在毛囊类器官中促进毛发生长的作用。然而，由于其分子量较大，其在体内迅速降解和较差的渗透性使其临床应用面临挑战。因此，在这项研究中，我们研究了催产素受体 (OXTR) 激动剂 WAY267464 和 LIT001 作为替代催产素对毛发生长的影响。我们将人真皮乳头 (DP) 细胞培养在添加 WAY267464 或 LIT001 的培养基中。WAY267464 和 LIT001 的添加增加了 DP 细胞中与毛发生长相关的基因的表达。我们使用体外毛囊类器官测试了 WAY267464 和 LIT001 对毛发生长的促进作用，发现它们显著促进了毛囊发芽。因此，我们的研究结果表明 WAY267464 和 LIT001 是潜在的毛发生长剂，可能会鼓励进一步研究针对 OXTR 的新型毛发生长剂在脱发患者中的开发。
