Pan Jia, Zhang Lihan, Liu Jie, Liang Yuanyuan
Institute of Pharmacology and Toxicology of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences No. 51, Section 4, Renmin South Road, Chengdu 610041, Sichuan, China.
School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine No. 37, Shierqiao Road, Chengdu 610075, Sichuan, China.
Am J Transl Res. 2024 Sep 15;16(9):4549-4563. doi: 10.62347/GEEW4432. eCollection 2024.
To investigate the effects of Jiannao pills on mice with chronic restraint stress-induced anxiety and its mechanisms.
Anxiety-like behaviors were induced in mice by exposing them to chronic restraint stress (8 h/day for 21 days). Subsequently, Jiannao pills were given to these mice for the treatment of the induced anxiety. Following treatment, the intestinal microflora of the mice were analyzed using the 16S rRNA sequencing method.
Under positive electrospray ionization mode, a total of 68 chemical compositions were found in Jiannao pills, and under negative electrospray ionization mode, the number was 18. With these chemical compositions in effect, it was observed that Jiannao pills alleviated chronic restraint induced anxiety-like behaviors in mice by extending their dwelling time, standing time, and grooming time in the central area, as well as the percentage of entries and time spent in the open arms. This effect was similar to that of alprazolam. In addition, Jiannao pills significantly improved neural functions in mice with chronic restraint-induced anxiety, diminished the levels of 5-hydroxytryptamine and glutamate, and increased the levels of γ-aminobutyric acid. Furthermore, Jiannao pills decreased the expressions of the corticotropin-releasing factor and cholecystokinin protein, while elevating neuropeptide Y protein levels. The results of 16S rRNA sequencing analysis revealed both Jiannao pills and alprazolam altered the composition of intestinal microflora in mice, with Jiannao pills exhibiting a more pronounced effect. Specifically, there was a significant increase in the abundance of S24-7 in mice following treatment. Besides, significant differences were observed in a total of 632 operational taxonomic units in mice after Jiannao pill treatment. The functions of the intestinal microflora of mice were primarily associated with their betalain biosynthesis and classification levels.
Jiannao pills effectively ameliorated chronic restraint anxiety-like behaviors in mice and enhanced their neural functions potentially through the regulation of their intestinal microflora.
探讨健脑丸对慢性束缚应激诱导的焦虑小鼠的影响及其作用机制。
通过对小鼠进行慢性束缚应激（每天8小时，持续21天）诱导其出现焦虑样行为。随后，给予这些小鼠健脑丸治疗诱导的焦虑。治疗后，采用16S rRNA测序方法分析小鼠的肠道微生物群。
在正电喷雾电离模式下，健脑丸中共发现68种化学成分，在负电喷雾电离模式下，该数字为18种。在这些化学成分的作用下，观察到健脑丸通过延长小鼠在中央区域的停留时间、站立时间和梳理时间，以及进入开放臂的次数和在开放臂中花费的时间百分比，减轻了慢性束缚诱导的焦虑样行为。这种效果与阿普唑仑相似。此外，健脑丸显著改善了慢性束缚诱导的焦虑小鼠的神经功能，降低了5-羟色胺和谷氨酸水平，并提高了γ-氨基丁酸水平。此外，健脑丸降低了促肾上腺皮质激素释放因子和胆囊收缩素蛋白的表达，同时提高了神经肽Y蛋白水平。16S rRNA测序分析结果显示，健脑丸和阿普唑仑均改变了小鼠肠道微生物群的组成，健脑丸的作用更为明显。具体而言，治疗后小鼠中S24-7的丰度显著增加。此外，健脑丸治疗后小鼠中总共632个可操作分类单元存在显著差异。小鼠肠道微生物群的功能主要与其甜菜碱生物合成和分类水平有关。
健脑丸可有效改善小鼠慢性束缚焦虑样行为，并可能通过调节其肠道微生物群增强其神经功能。
