Health Care & Nutritional Science Institute, R&D Division, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama 252-8583, Japan.
Nutrients. 2024 Oct 2;16(19):3347. doi: 10.3390/nu16193347.
The gut microbiota of breast-fed infants is dominated by infant-type human-residential bifidobacteria (HRB) that contribute to infant health; thus, it is crucial to develop infant formulas that promote the establishment of a gut microbiota enriched with infant-type HRB, closely resembling that of breastfed infants.
We compared various non-digestible prebiotic oligosaccharides and their combinations using a fecal culture system to explore which candidates could promote the growth of all infant-type HRB and rarely yield non-responders. The analysis included lactulose (LAC), raffinose (RAF), galactooligosaccharides (GOS), and short- and long-chain fructooligosaccharides. Fecal samples were collected from seven infants aged 1.5-10.2 months and cultured with each oligosaccharide individually or their combinations.
No single oligosaccharide effectively promoted the growth of all infant-type HRB, although GOS promoted the growth of HRB other than subsp. . Only the LAC/RAF/GOS group evenly and effectively promoted the growth of all infant-type HRB. Accordingly, acetate production was higher in fecal cultures supplemented with GOS or LAC/RAF/GOS than in the other cultures, suggesting that it is a superior combination for all infant-type HRB and rarely yields non-responders.
This study can aid in developing infant formulas that help align the gut microbiota of formula-fed infants with that of breastfed infants.
母乳喂养婴儿的肠道微生物群主要由婴儿型人居住双歧杆菌（HRB）主导，这些细菌有助于婴儿健康；因此，开发能够促进富含婴儿型 HRB 的肠道微生物群建立的婴儿配方奶粉至关重要，使其与母乳喂养婴儿的肠道微生物群非常相似。
我们使用粪便培养系统比较了各种不可消化的益生元低聚糖及其组合，以探索哪些候选物可以促进所有婴儿型 HRB 的生长，并且很少产生无应答者。分析包括乳果糖（LAC）、棉子糖（RAF）、半乳糖低聚糖（GOS）以及短链和长链果聚糖。从 7 名 1.5-10.2 月龄的婴儿中收集粪便样本，并分别或组合培养每种低聚糖。
虽然 GOS 促进了除 subsp. 以外的 HRB 的生长，但没有一种单独的低聚糖能有效地促进所有婴儿型 HRB 的生长。只有 LAC/RAF/GOS 组能均匀有效地促进所有婴儿型 HRB 的生长。因此，在补充 GOS 或 LAC/RAF/GOS 的粪便培养物中乙酸盐的产生高于其他培养物，表明这是一种对所有婴儿型 HRB 都有效的组合，很少产生无应答者。
本研究可以帮助开发有助于使配方喂养婴儿的肠道微生物群与母乳喂养婴儿的肠道微生物群相匹配的婴儿配方奶粉。
