Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
National Reference Laboratory of Tuberculosis, Department of Microbiology, National Center of Infectious and Parasitic Diseases, 44A General Nikolai Stoletov Boulevard, 1233 Sofia, Bulgaria.
Int J Mol Sci. 2024 Sep 27;25(19):10434. doi: 10.3390/ijms251910434.
We investigated the rise of nontuberculous mycobacteria (NTM) infections in Bulgaria, focusing on species identification and distribution from 2018 to 2022. Utilizing advanced diagnostic tools, including the Hain Mycobacterium CM/AS method, Myco-biochip assay, and whole-genome sequencing, the study identifies and characterizes a diverse range of species from clinical samples. While , , , and were dominating, a number of rare species were also found. They include such species as and . Moreover, the noticeable prevalence of complex species missed by conventional testing was observed. We identified a rare species, highly homologous to previously described strains from Japan; based on genome-genome distance data, we propose its reannotation as a new species. Further, a novel species was identified, which is significantly distinct from its closest neighbor, with ANI = 87.18%. Based on the SeqCode procedure, we propose to name this new species sp. nov. Dynamic changes in NTM species prevalence in Bulgaria observed from 2011 to 2022 highlight the emergence of new species and variations tied to environmental and demographic factors. This underscores the importance of accurate species identification and genotyping for understanding NTM epidemiology, informing public health strategies, and enhancing diagnostic accuracy and treatment protocols.
我们调查了保加利亚非结核分枝杆菌（NTM）感染的上升情况，重点关注 2018 年至 2022 年期间从临床样本中鉴定和分布的物种。本研究利用先进的诊断工具，包括 Hain Mycobacterium CM/AS 方法、Myco-biochip 检测和全基因组测序，鉴定和描述了来自临床样本的多样化的物种。虽然 、 、 和 占主导地位，但也发现了一些罕见的物种。它们包括 和 等物种。此外，还观察到常规检测遗漏的 复合体物种的明显流行。我们鉴定了一个罕见的物种，与之前描述的来自日本的菌株高度同源；根据基因组-基因组距离数据，我们建议将其重新注释为一个新物种。此外，还鉴定了一个新的物种，与最近的邻居 具有明显的差异，ANI=87.18%。根据 SeqCode 程序，我们建议将这个新物种命名为 sp. nov. 从 2011 年到 2022 年，保加利亚 NTM 物种流行率的动态变化突出了与环境和人口因素有关的新物种和变异的出现。这强调了准确的物种鉴定和基因分型对于理解 NTM 流行病学、为公共卫生策略提供信息、提高诊断准确性和治疗方案的重要性。
