Xia Zetian, Mi Haifeng, Ren Mingchun, Huang Dongyu, Aboseif Ahmed Mohamed, Liang Hualiang, Zhang Lu
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Tongwei Agricultural Development Co., Ltd., Key Laboratory of Nutrition and Healthy Culture of Aquatic, Livestock and Poultry, Ministry of Agriculture and Rural Affairs, Healthy Aquaculture Key Laboratory of Sichuan Province, Chengdu 610093, China.
Animals (Basel). 2024 Oct 5;14(19):2871. doi: 10.3390/ani14192871.
This experiment evaluated the function of chlorogenic acid (CGA) in the growth, health status, and inflammation of largemouth bass (). Over eight weeks, CGA supplementation was designed at five levels: 0, 60, 120, 180, and 240 mg/kg. The 180 and 240 mg/kg CGA-supplemented groups showed significant improvements in the FBW, SGR, and WGR compared to the control group (0 mg/kg) ( < 0.05). All the CGA-supplemented groups exhibited a significant reduction in the FCR ( < 0.05), with the 180 mg/kg CGA group showing the lowest FCR. Nonetheless, there were no appreciable differences in the plasma concentrations of TP, ALT, or AST among the treatments ( > 0.05). Compared to the control group, the 180 mg/kg CGA group exhibited significantly lower TC and TG levels ( < 0.05). The ALP levels showed no significant differences from the control group ( > 0.05). In terms of antioxidant parameters, CGA supplementation considerably reduced the MDA content ( < 0.05) and increased the GSH levels, while decreasing the , , and activity levels Meanwhile, CGA supplementation resulted in reduced mRNA levels of , , , , and compared to the control group. In contrast, the mRNA levels of , , , and were elevated in the liver. Our findings indicated that CGA supplementation improved the growth performance and antioxidant status and weakened the inflammatory response of largemouth bass. These findings suggest that CGA could be a valuable dietary supplement for enhancing the health and growth of this species.
本实验评估了绿原酸（CGA）对大口黑鲈生长、健康状况和炎症的作用。在八周时间里，将CGA补充剂设计为五个水平：0、60、120、180和240毫克/千克。与对照组（0毫克/千克）相比，补充180和240毫克/千克CGA的组在终末体重（FBW）、特定生长率（SGR）和增重率（WGR）方面有显著改善（P<0.05）。所有补充CGA的组饲料系数（FCR）均显著降低（P<0.05），其中180毫克/千克CGA组的FCR最低。然而，各处理组之间总蛋白（TP）、谷丙转氨酶（ALT）或谷草转氨酶（AST）的血浆浓度没有明显差异（P>0.05）。与对照组相比，180毫克/千克CGA组的总胆固醇（TC）和甘油三酯（TG）水平显著降低（P<0.05）。碱性磷酸酶（ALP）水平与对照组无显著差异（P>0.05）。在抗氧化参数方面，补充CGA可显著降低丙二醛（MDA）含量（P<0.05）并提高谷胱甘肽（GSH）水平，同时降低超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性水平。同时，与对照组相比，补充CGA导致白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、环氧合酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的mRNA水平降低。相反，肝脏中白细胞介素-10（IL-10）、转化生长因子-β1（TGF-β1）、核因子E2相关因子2（Nrf2）和血红素加氧酶-1（HO-1）的mRNA水平升高。我们的研究结果表明，补充CGA可改善大口黑鲈的生长性能和抗氧化状态，并减弱其炎症反应。这些发现表明，CGA可能是一种有价值的饲料添加剂，可促进该物种的健康和生长。
