Beachey E H
Infect Immun. 1986 Jan;51(1):362-4. doi: 10.1128/iai.51.1.362-364.1986.
A chemically synthesized copy (S-CB7) of a fragment (35 amino acid residues) of type 24 streptococcal M protein was covalently linked to polylysine with carbodiimide and injected subcutaneously into rabbits without adjuvant. Although the primary immune responses as measured by enzyme-linked immunosorbent assays at biweekly intervals were weak, the secondary responses as measured by both enzyme-linked immunosorbent assays and opsonophagocytic assays were as high as those obtained previously in rabbits immunized with the peptide conjugate emulsified in complete Freund adjuvant. Injection of murabutide, a synthetic muramyl dipeptide derivative of bacterial peptidoglycan, with the initial immunizing dose of peptide conjugate had no apparent effect on the secondary immune responses. These results indicate that protective immune responses may be raised against polylysine conjugates of chemically synthesized peptide copies of streptococcal M protein without adjuvant.
将24型链球菌M蛋白片段（35个氨基酸残基）的化学合成副本（S-CB7）用碳二亚胺与聚赖氨酸共价连接，并在无佐剂的情况下皮下注射到兔子体内。尽管每两周通过酶联免疫吸附测定法测量的初次免疫反应较弱，但通过酶联免疫吸附测定法和调理吞噬测定法测量的二次反应与之前用完全弗氏佐剂乳化的肽缀合物免疫的兔子所获得的反应一样高。在初次免疫剂量的肽缀合物中注射murabutide（一种细菌肽聚糖的合成胞壁酰二肽衍生物）对二次免疫反应没有明显影响。这些结果表明，在无佐剂的情况下，针对链球菌M蛋白化学合成肽副本的聚赖氨酸缀合物可能会引发保护性免疫反应。
