Department of Pathology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra, India.
J Cancer Res Ther. 2024 Jul 1;20(5):1390-1393. doi: 10.4103/jcrt.jcrt_9_23. Epub 2023 May 3.
Carcinogenesis is associated with multiple mutations that lead to chromosomal instability (CIN). Our aim was to study the role of markers of CIN on breast cytology and correlate with histopathological diagnosis.
A retrospective observational study was conducted over a year on 100 breast cytology cases. Leishman-stained smears were examined for the number of micronuclei (MN), chromatin bridge (CB), and multipolar mitosis (MPM). MNs were counted manually in 1,000 cells under oil immersion (1000×). The frequency of CB and MPM was also noted. The malignant cases were graded as low (grades 1 and 2) and high (grade 3).
The mean age of the patients was 43.47 ± 15.81 years. The mean MN score for fibroadenoma cases was 4.54 (±1.5) and for malignant cases was 29.32 (±8.15). The receiver-operator curve (ROC) curve analysis confirmed >9 MN score as 100% specific and 100% sensitive for malignancy (area under the curve [AUC] =1). A significant comparison was noted between the mean MN of benign and malignant cases (P < 0.000001). The mean MN score for low-grade cases was 26.64 (±5.85) and for high-grade cases was 33.68 (±5.85) with a significant difference observed on comparing the population mean MN with a grade of malignancy (P-value = 0.01886). The difference between the frequencies of CB in low and high-grade cases was also found to be significant (P-value = 0.04135).
The presence and scoring of markers of CIN (MN, CB, and MPM) on breast cytology have potential significance in the diagnosis of breast lesions.
癌变与导致染色体不稳定（CIN）的多种突变有关。我们的目的是研究 CIN 标志物在乳腺细胞学中的作用，并与组织病理学诊断相关联。
对 100 例乳腺细胞学病例进行了为期一年的回顾性观察性研究。用 Leishman 染色的涂片检查微核（MN）、染色质桥（CB）和多极有丝分裂（MPM）的数量。在油浸（1000×）下手动计数 1000 个细胞中的 MN，计算 MN 数量。还记录了 CB 和 MPM 的频率。恶性病例分为低（1 级和 2 级）和高（3 级）。
患者的平均年龄为 43.47 ± 15.81 岁。纤维腺瘤病例的平均 MN 评分 4.54（±1.5），恶性病例的平均 MN 评分为 29.32（±8.15）。接收者操作特征（ROC）曲线分析证实>9 MN 评分对恶性肿瘤具有 100%的特异性和 100%的敏感性（曲线下面积 [AUC] =1）。良性和恶性病例的平均 MN 之间存在显著差异（P < 0.000001）。低级别病例的平均 MN 评分为 26.64（±5.85），高级别病例的平均 MN 评分为 33.68（±5.85），对恶性肿瘤的人群平均 MN 评分进行比较，观察到有显著差异（P 值 = 0.01886）。低级别和高级别病例 CB 频率之间的差异也具有统计学意义（P 值 = 0.04135）。
在乳腺细胞学中 CIN 标志物（MN、CB 和 MPM）的存在和评分可能对乳腺病变的诊断具有重要意义。
