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使用差示扫描荧光法体外评估蛋白质 - 多氟烷基物质的方案

Protocol for evaluating protein-polyfluoroalkyl substances in vitro using differential scanning fluorimetry.

作者信息

Starnes Hannah M, Belcher Scott M

机构信息

Department of Biological Sciences, North Carolina State University, Raleigh, NC 27695, USA.

Department of Biological Sciences, North Carolina State University, Raleigh, NC 27695, USA.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103386. doi: 10.1016/j.xpro.2024.103386. Epub 2024 Oct 15.

DOI:10.1016/j.xpro.2024.103386
PMID:39412995
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11530897/
Abstract

Per- and polyfluoroalkyl substances (PFAS) are ubiquitous synthetic chemicals that threaten public health, and serum albumin binding of PFAS represents one major variable influencing PFAS toxicokinetics. In this protocol, we describe a differential scanning fluorimetry (DSF) assay suitable for the rapid determination of the relative binding affinities of serum albumin proteins to different PFAS. Herein, we address common experimental challenges related to PFAS solubility constraints, the high background fluorescence of DSF with serum albumins, and the limitations of using DSF-derived dissociation constants (K) to quantify PFAS-albumin interactions. For complete details on the use and execution of this protocol, please refer to Jackson et al..

摘要

全氟和多氟烷基物质（PFAS）是普遍存在的合成化学品，威胁着公众健康，而PFAS与血清白蛋白的结合是影响PFAS毒代动力学的一个主要变量。在本方案中，我们描述了一种差示扫描荧光法（DSF）测定方法，适用于快速测定血清白蛋白与不同PFAS的相对结合亲和力。在此，我们解决了与PFAS溶解度限制、DSF与血清白蛋白的高背景荧光以及使用DSF衍生的解离常数（K）来量化PFAS-白蛋白相互作用的局限性相关的常见实验挑战。有关本方案使用和执行的完整详细信息，请参考杰克逊等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/3ef331cc3b73/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/9306db06adab/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/132cc7bc2771/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/f3ea930975d2/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/8d704b56636d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/f9ea19863342/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/8e6fd2624712/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/af14540cab86/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/3ef331cc3b73/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/9306db06adab/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/132cc7bc2771/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/f3ea930975d2/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/8d704b56636d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/f9ea19863342/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/8e6fd2624712/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/af14540cab86/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/11530897/3ef331cc3b73/gr7.jpg

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本文引用的文献

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2
In Vitro and In Silico Analysis of the Bindings between Legacy and Novel Per- and Polyfluoroalkyl Substances and Human Serum Albumin.传统与新型全氟和多氟烷基物质与人血清白蛋白结合的体外和计算机模拟分析
Toxics. 2024 Jan 8;12(1):46. doi: 10.3390/toxics12010046.
3
Protocol for performing and optimizing differential scanning fluorimetry experiments.
进行和优化差示扫描荧光法实验的方案。
STAR Protoc. 2023 Dec 15;4(4):102688. doi: 10.1016/j.xpro.2023.102688. Epub 2023 Nov 8.
4
Epidemiology Evidence for Health Effects of 150 per- and Polyfluoroalkyl Substances: A Systematic Evidence Map.流行病学证据表明 150 种全氟和多氟烷基物质对健康的影响：系统证据图谱。
Environ Health Perspect. 2022 Sep;130(9):96003. doi: 10.1289/EHP11185. Epub 2022 Sep 30.
5
Investigation of the Interaction between Human Serum Albumin and Branched Short-Chain Perfluoroalkyl Compounds.研究人血清白蛋白与支链短链全氟烷基化合物的相互作用。
Chem Res Toxicol. 2022 Nov 21;35(11):2049-2058. doi: 10.1021/acs.chemrestox.2c00211. Epub 2022 Sep 23.
6
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Int J Environ Res Public Health. 2020 Feb 18;17(4):1319. doi: 10.3390/ijerph17041319.