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磁性微型机器人作为细胞清理平台

Magnetic Microrobots as a Platform for Cell Clean Up.

作者信息

Kirmizitas Fatma Ceren, Rivas David, Mallick Sudipta, DePope Scott, Das Sambeeta

机构信息

Department of Mechanical Engineering, University of Delaware, Newark, DE 19716.

Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716.

出版信息

Int Conf Manip Autom Robot Small Scales. 2023 Oct;2023. doi: 10.1109/marss58567.2023.10294141. Epub 2023 Oct 31.

Abstract

Mobile magnetic microrobots have been extensively used in a wide range of biomedical applications due to their numerous advantages. Magnetic microrobots in particular have been developed and shown great potential over the past two decades for the manipulation and migration of both single cells and cell aggregates. The efficient clearance of cell aggregates is crucial to prevent uncontrolled cell proliferation, tissue damage, and invasive surgeries, especially for those related to the vascular system. In this work, we showed cellular manipulation and mobility to achieve cellular clean up on Human Liver Cancer (HepG2) cells by using two types of untethered magnetic microrobots that are different in type and size. We performed the cellular clean up in the microchannel, which can demonstrate the closed working environment, and also on a glass slide to present an air-liquid interface. We showed that the microrobots could be able to move a cluster of cells in both conditions which could make them useful for sorting and separation applications. Furthermore, cell viability was assessed by using a trypan blue staining assay on HepG2 cells right after and 24 hours after microrobot actuation.

摘要

由于具有众多优点,移动磁性微型机器人已广泛应用于各种生物医学领域。特别是在过去二十年中,磁性微型机器人得到了发展,并在单细胞和细胞聚集体的操纵与迁移方面显示出巨大潜力。细胞聚集体的有效清除对于防止细胞不受控制地增殖,并避免组织损伤和侵入性手术至关重要,尤其是对于那些与血管系统相关的手术。在这项工作中,我们展示了通过使用两种类型和尺寸不同的无系绳磁性微型机器人,对人肝癌(HepG2)细胞进行细胞操纵和移动,以实现细胞清理。我们在微通道中进行了细胞清理,微通道可展示封闭的工作环境,并且也在载玻片上进行了清理,以呈现气液界面。我们表明,在这两种条件下微型机器人都能够移动细胞簇,这使得它们可用于分选和分离应用。此外,在微型机器人启动后立即以及启动24小时后,通过台盼蓝染色法对HepG2细胞的活力进行了评估。

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